党参药材中党参炔苷与总多糖总皂苷含量相关性研究.docVIP

党参药材中党参炔苷与总多糖总皂苷含量相关性研究 　　 　　摘 要：目的：研究不同产地、不同规格党参药材中党参炔苷、总多糖、总皂苷的含量，对其测定结果进行比较，分析三者之间的相关性，为评价和控制党参药材质量提供参考依据。方法：采用HPLC法测定党参炔苷，采用硫酸-苯酚比色法测定总多糖成分，采用香草醛-高氯酸比色法测定总皂苷。结果：党参炔苷在0.0085~0425mg.mL-1(r=0.9998)范围内与峰面积呈良好线性关系，精密度良好，平均回收率100.7%；总多糖、总皂苷测定精密度及回收率亦符合要求。测定了5个不同产地、9种规格的党参药材样品。结论：党参样品中党参炔苷与总皂苷含量相关，与总多糖不成正比，这为科学评价和有效控制其质量提供了依据。 　　关键词：党参；党参炔苷；相关性 　　 中图分类号：R28文献标识码：A文章编号：1673-7717(2011)12-2779-03 　　Correlation of Lobetyolin,Total Polysaccharides and Total Saponins from Different 　　Region of Radix Codonopsis 　　SHI Jun1，MA Fangli2，WANG Xianyan1，HUANG Sihang1 　　(1.Guangdong Pharmacy University,Guangzhou Higher Education Mega Centre,Guangzhou 510006， 　　Guangdong，China;2.Infinitus (China) Company Ltd., Guangzhou 510665，Guangdong，China) 　　 Abstract:Objective: To determine content of lobetyolin and total polysaccharides and total saponins from different region of Radix Codonopsis, and to explore the correlation between them so as to control the quality of Radix Codonopsis. Methods：Diamonsil C18 column (4.6mm×25cm,5μm) were used, the mobile phase was acetonitrile-water(25∶ 75) and the UV detector was set at 268nm. Use Colormetric method was used to determine the total polysaccharides and total saponins content. Results：The calibration curve was linear in the range of 0.0085~0.425mg.mL-1(r=0.9998),the average recovery ratio was 100.7%. The contents of lobetyolin in codonopsis from 5 habits were detected between 0.187mg/g and 1.314 mg/g. The precision and recovery ratio fits the requirement. Conclusions: There was certain correlation between lobetyolin and total saponins, but total polysaccharides not. 　　 Key words:Radix Codonopsis; Lobetyolin; HPLC 　　________________________________________ 　　收稿日期：2011-07-25 　　作者简介：时军（1980-），男，讲师，研究方向：中药药剂学。党参为补益类常用中药，味甘、性平，入脾、肺二经，具有补中益气、生津和胃之功效\[1\]。党参主产于山西、甘肃、四川、云南、湖北等地，目前市场上多为栽培品种。其近年来，党参广泛用于治疗冠心病、高血压、高血脂、低血压病和功能性子宫出血等症，作为食物同源的中药，党参在保健食品方面亦有很广的应用\[2\]。为进行党参药材的质量评价，本文采集国内主流品种的党参药材，进行党参炔苷、总多糖、总皂苷的含量测定，以期为党参药材的质量评价及合理应用提供参考\[3-4