第18届国际奥林匹克竞赛生物竞赛试题和的答案.docVIP

18th INTERNATIONAL BIOLOGY OLYMPIAD July 15 – 22, 2007 第18届国际生物奥林匹亚 PRACTICAL EXAM 4 实作4 GENETICS 遗传学 【会先进行色盲检验】 TASK A. Sequence confirmation of a cDNA 23 marks 一段cDNA序列的组成 (20分) TASK B. Genetics of coat colour in dogs 16 marks TASK C. Genetic control of seed coat colour and seed shape in beans 20 marks 豆子种皮颜色及种子性状遗传的控制 (20分) Time allowed: 90 minutes 共90分钟 WRITE ALL ANSWERS IN THIS EXAM BOOKLET. 所有答案要写在这本试卷上 WRITE YOUR 4-DIGIT STUDENT CODE IN THE BOX BELOW AND ON THE TOP OF EACH PAGE OF THIS BOOKLET 将你的4码学生代码写在下栏中并写在其它各页的上方 STUDENT CODE 学生代码 STUDENT CODE _____________________ 学生代码 TASK A. Sequence Confirmation of a cDNA (23 marks) 一段cDNA序列的组成(23分) Objective: To isolate plasmid DNA containing a cDNA of interest and to determine the sequence of the cDNA. 目的：分离一段含有待研究之cDNA的质体DNA，并决定此cDNA的序列 Introduction: To over-express a gene of interest in a plant or animal you must first isolate the gene of interest in the form of a cDNA. You have done this and in order to amplify this DNA, you have cloned it into the pBluescript SK plasmid vector which you have subsequently used to transform bacteria cells. You must now carry out a quick plasmid preparation to isolate the plasmid and confirm the sequence of your cDNA insert. 介绍：要过度表现植物或动物有待研究的一个基因，你必须先以cDNA的形式分离出这段基因；若你已完成分离，要放大这段DNA并clone在pBluescript SK质体的载体中，接着用这载体来转形细菌；你必须快速进行质体制备，以分离质体并确定此cDNA插入段的序列。 Materials 材料Quantity (数)量 Bacterial cell culture 4 mL 细菌培养液 1.5 mL microcentrifuge tubes 5 1.5 mL 微离心管 Microcentrifuge rack 1 微离心管架 P1000 micropipettor 1 P1000微吸管 Box of 200-1000 uL pipette tips 1 200-1000 uL吸管尖盒 GET buffer (1.5 mL tube) 0.5 mL GET缓冲液(1.5 mL小离心管) 10% Sodium Dodecyl Sulphate (1.5 mL tube) 0.5 mL 10% SDS (1.5 mL小离心管) 2 N NaOH (1.5 mL tube) 0.5 mL 2 N NaOH (1.5 mL小离心管) 3 M Potassium 5 M Acetate (1.5 mL tube) 0.5 mL 3 M 钾 5 M 醋酸 (1.5 mL小离心管) 95% ethanol (Falcon tube) 3 mL 95% 乙醇 (试管) Distilled water (Falcon tube) 3 mL 蒸馏水 (试管) Timer 1 定时器 Tube labels 2 试管标签 Marker pen 1 签字笔