真菌DNA提取方法的建立与比较.docVIP

真菌DNA提取方法的建立和比较**☆○ 杨艳秋1，王 丽2，贺 丹2，孙文通2，张 宇2，横山耕治○3 ? 1吉林大学第一医院, 吉林省长春市　130021；2吉林大学基础医学院病原生物学教研室, 吉林省长春市 130021；3日本国立千叶大学真菌医学研究中心, 日本千叶 260-8673 杨艳秋☆，女，1961年生，吉林省长春市人，汉族，2007年吉林大学基础医学院病毕业，博士，副教授，主要从事真菌学研究。8634469@ 通讯作者：王 丽，教授，吉林大学基础医学院病原生物学教研室，吉林省长春市 130021 wli99@ 国家自然科学基金资助项目*；吉林省科技发展计划重点资助项目3）* 中图分类号:R519 文献标识码:A文章编号:1673-8225(2007)50-10093-04 收稿日期：2007-09-06修回日期：2007-10-06(07-50-9-4864/GW·Q) ? ? ? ? ? Comparison of three methods of genomic DNA extraction from fungus ＡｂｓｔｒａｃｔＡＩＭ:The DNA extraction rate, especially the DNA purity, has a strong influence on the experiment results. There is a big difference of purity and extraction rate in extracting DNA either towards the same sample with the different method or towards the different samples with the same method. In this study, in order to find the best DNA extraction method, we used filamentous fungi as the sample and optimized the method. ＭＥＴＨＯＤＳ:This study was carried out at Mycology Lab of Basic Medical College of Jilin University from June 2004 to December 2006. Using clinical common pathomycete as the samples, we took three methods, including glass-bead salt fractionation, CTAB and Gene TLETM, to extract DNA and compared the extraction rate via agarose gel electrophoresis method.ＲＥＳＵＬＴＳ:Under the same condition, the ratio between A260 and A280 of Gene TLETM was the highest, which presented the protein content was relatively low. Comparing the DNA agarose gel electrophoretogram made by the three methods, all the DNA can amplify the aim fragment in multi-PCR and the DNA extracted by Gene TLETM showed the best result.ＣＯＮＣＬＵＳＩＯＮ:Gene TLETM is suit to extract DNA and has the advantages of high extraction rate, good quality and simple operation. Yang YQ, Wang L, He D, Sun WT, Zhang Y, Hengshan GZ.Comparison of three methods of genomic DNA extraction from fungus.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2007;11(50):10093-10096(China) [/zglckf/ejournal/upfiles/07-50/