Design of UltraHigh Density DNALaden Polylysinemodified 设计超高密度聚赖氨酸修饰DNA拉登.pptVIP

下载本文档

2
0
约4.8千字
约 12页
2018-06-15 发布于福建
举报
版权申诉

Design of UltraHigh Density DNALaden Polylysinemodified 设计超高密度聚赖氨酸修饰DNA拉登.ppt

1、本文档共12页，可阅读全部内容。
2、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Design of UltraHigh Density DNALaden Polylysinemodified 设计超高密度聚赖氨酸修饰DNA拉登

Design of Ultra-High Density DNA-Laden Polylysine-modified Silicon Surfaces Presented by Michael D. Soriano Graduate Mentor: Yuli Wang Faculty Mentor: Dr. Ying C. Chang UC LEADS Program August 15, 2002 Abstract Single-stranded DNA was attached to polylysine-modified silicon (PLS) surfaces. Initially, the PLS substrate was protected. Using a mixture of HBr/CH3COOH, the surface was deprotected, ready for the coupling reaction with the natural DNA, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDAC), and N-hydroxysuccinimide (NHS). Measurements of the characterization of each surface were taken: the contact angle, refractive index, and thickness before and after deprotection; thickness and refractive index after the coupling reactions. The coupling reactions were optimized by modifying the surface density of the PLS. Binding specificity of complementary DNA was tested for using fluorescence hybridization. Preliminary results showed increases in substrate thickness from 20-80% after the coupling reactions. Introduction The current technology of DNA microarrays attaches the nucleotides via bi-hetero-functional linkers1 or heat/light2, e.g. ultraviolet (UV) light. Protocols for microarray design call for coating silicon or glass slides with polylysine (PLL), then DNA attachment is mediated. The resulting microarrays may effectively only be used once and have a superficial coating of DNA on the surfaces. Lenigk et al.3 have created microarrays for repeated use by using (3-mercaptopropyl)trimethoxysilane. They have successfully shown that their design may be used repeatedly up to five times. Our design implements attachment of DNA strands to polylysine substrates. We have effectively increased the thickness of the substrate from 20 – 80% after coupling reactions. Background fluorescence contaminated the sample Materials/MethodsDeprotection of Substrates Protected substrates according to Wang4 Substrates were suspended in a layer of benze