利用SCE技术对细胞期分析方法的研究毕业论文.doc

华中科技大学文华学院 毕 业 设 计（论文） 题目（中文）：利用SCE技术对细胞周期分析方法的研究 Using the technology of SCE analysis methods of the cell cycle 学 生 姓 名： 学号：060109011103 学 部 （系）： 信息科学与技术学部 专 业 班 级： 06生物技术1班 指 导 教 师： 目 录 摘要………………………………………………………………………………1 关键词…………………………………………………………………………1 Abstract…………………………………………………………………………2 Key words………………………………………………………………………2 1 . 选题背景……………………………………………………………………1 1.1 课题来源…………………………………………………………………………1 1.2 国内外的相关研究……………………………………………………………1 1.3 目的和意义………………………………………………………………………1 1.4 课题设计指导思想……………………………………………………………5 2 . 方案论证……………………………………………………………………7 2.1方案的设计原理………………………………………………7 2.2 方案论证……………………………………………………7 3 . 材料与方法…………………………………………………………………13 3.1 材料与仪器……………………………………………………………………13 3.2 实验步骤…………………………………………………………………………13 4 . 结果与讨论…………………………………………………………………16 4.1 实验结果…………………………………………………………………………16 4.2 结果分析…………………………………………………………………………17 4.3 结果讨论…………………………………………………………………………18 5 . 结论与心得…………………………………………………………………20 5.1 结论………………………………………………………………………………20 5.2 心得………………………………………………………………………………20 致谢……………………………………………………………………………23 附录……………………………………………………………………………24 附－1 实验药品………………………………………………………………………24 附－2 实验仪器………………………………………………………………………24 参考文献………………………………………………………………………25 摘要 本文以BrdU-Giemsa技术检测研究了正常细胞和肿瘤细胞的细胞动力学参数和姐妹染色单体交换频率（SCE）bstract： Based on BrdU - Giemsa technology is studied and the normal cell detection of cell tumor cell kinetics parameters and sister chromatids switching frequency (SCE) frequency. Sister chromatids exchange (SCE) sister chromatid exchange technology is developed rapidly since the 1970s and is widely accepted a screening chemical mutagenesis content short-term experimental methods. SCE said in chromosome chain of DNA duplicate process such sites, chromosomes happened in exchange of DNA level on stability, it is sensitive to the damage DNA damage, response and for many carcinogens or mutagenesis induced objects. Many laboratory, this method is as an imp