18β甘草次酸衍生物对smmc7721的影响毕业论文.doc

18β甘草次酸衍生物对smmc-7721的影响 Effects of 18β-glycyrrhetinic Acid Derivatives in SMMC-7721 cells 摘要：目的：研究18β-甘草次酸衍生物对SMMC-7721、细胞增殖的影响。方法：利用细胞计数法，倒置显微镜法，MTT法，oecHhst-33258染色法，流式细胞仪检测法，观察和研究经不同浓度18β-甘草次酸衍物作用24h，48h和72h的肝癌细胞SMMC-7721的形态结构，生长抑制率,细胞凋亡的变化，并探究了对SMMC-7721影响的最适18β-甘草次酸衍物的浓度。结论：经不同浓度的18β-甘草次酸衍物作用后的SMMC-7721，经显微镜观察显示:细胞的形态发生改变，出现膨胀或皱缩现象；通过MTT检测表明细胞的增殖抑制率随18β-甘草次酸衍物浓度的增加而增加，随着作用时间的增长而增加，通过，Hoechst-33258试剂盒染色法研究表明18β-甘草次酸衍物还可影响细胞的凋亡。 关键词：18β-甘草次酸衍物SMMC-7721 细胞增殖 细胞凋亡 细胞形态 Effects of 18β-glycyrrhetinic Acid Derivatives in SMMC-7721 cells Abstract: Purpose: Study the 18β-glycyrrhetinic Acid Derivatives to cancer of the liver the influence of cells. Methods: Using the method of microscopic to inverted microscope, MTT method, Hoechst-33258 Staining Kit. In order to observations and research the 18β-glycyrrhetinic Acid Derivatives training 24 h, 48 h and 72h of liver cancer cells SMMC-7721 the morphological structure, growth inhibition rate and the change of the cell apoptosis. And we will explore the effect of SMMC-7721 on the most suitable 18β-glycyrrhetinic acid derivative of concentration. Conclusion: the18β-glycyrrhetinic acid derivative cultured SMMC-7721, the microscope observation showed that: cell morphological changes, swelling or shrinkage phenomenon; detected by MTT showed that the inhibition rate of cell proliferation with the increase of 18 β - glycyrrhetinic acid derivative concentration increases with time, with the growth of increase, through, Hoechst-33258 kit staining showed that 18β-glycyrrhetinic acid derivative can also influencecellapoptosis Keywords:18 β - glycyrrhetinic acid derivative SMMC-7721 cells proliferation and apoptosi 目录 前 言 2 1.实验用品 5 1.1仪器 5 1.2试剂 5 2.试验方法 5 2.11640培养基的配置 5 2.1.1过滤器的准备和安装 5 2.1.21640培养基的配置 5 2.2细胞复苏 6 2.3细胞传代 6 2.4细胞冻存 7 2.5 利用MTT法检测18β-甘草次酸衍生物对肝癌细胞的抑制率 7 2.5.1试剂配制 8 2.5.2细胞计数 8 2.5.3利用MTT法检