弗氏柠檬酸杆菌TPL因的体外扩增毕业论文.docVIP

PAGE PAGE 1 常州工程职业技术学院 毕业设计报告（论文） 系 别： 制药与生物工程技术系 课题名称： 弗氏柠檬酸杆菌TPL基因的体外扩增 指导教师： 班 级： 生物制药1021 学生姓名： 摘 要聚合酶链式反应(Polymerase Chain Reaction，简称PCR)是最常用的分子生物学技术之一，通过变性、退火和延伸的循环来完成核酸分子的大量扩增。本实验是以基因组 DNA 为模板，利用PCR 技术从弗氏柠檬酸杆菌( Ci trobacter f reundii ) 中扩增得到含有酪氨酸酚解酶（the Tyrosine Phenol Lyase，简称TPL）基因的有效DNA 片段，得到大量的有效基因。PCR技术产生时间虽不长，却以惊人的速度广泛应用于分子生物学各领域。PCR技术能够快速特异地扩增任何目的基因或DNA片段，它不仅可以用于基因分离、克隆和核酸序列分析，还可用于突变体和重组体的构建、基因表达调控的研究、基因多态性的分析、遗传病和传染病的诊断、肿瘤机制探查、法医鉴定等诸多方面。PCR技术已成为方法学上的一次革命，它必将大大推动分子生物学各学科和研究进展。关键词：弗氏柠檬酸杆菌 TPL 基因 PCR 扩增AbstractPolymerase Chain Reaction (PCR) is one of the common techniques in molecular biology, which can amplify nucleic acids through the cycle of denaturation， annealing and extension. Tyrosine phenol lyase gene-containing DNA fragment was amplified from Citrobacter freundii, get a lot of effective gene using PCR technology. PCR technology generation time is not long, but it is widely used in various fields of molecular biology at an alarming rate. PCR technology can quickly and specifically any desired gene or DNA fragment was amplified, It not only can be used for gene isolation, cloning and DNA sequencing analysis can also be used for the construction of mutant and recombinant, the study of the regulation of gene expression, gene polymorphism analysis, genetic disease and infectious disease diagnosis, tumor mechanism explorationThe forensic identification, and many other aspects. PCR technology has become a revolution on the methodology, it will greatly promote the various disciplines of molecular biology and research progress.Key words：citrobacter freundii gene PCR TPL amplification常州工程职业技术学院毕业设计（论文）共 17页 第 PAGE \* MERGEFORMAT 17页目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc346115402 1 前言 PAGEREF _Toc346115402 \h 2 HYPERLINK \l _Toc346115403 1.1络氨酸酚裂解酶 PAGEREF _Toc34611540