山柰酚在sd大鼠体内的药代动力学及其代谢分析-pharmacokinetics and metabolic analysis of kaempferol in sd rats.docx

Mobile phase: acetonitrile: water (plus 0.2% phosphoric acid) gradient elution and gradient range (20%-40% acetonitrile) elution 40min. Velocity: 1ml/min, wavelength: 360nm, column temperature: 40 ℃, incoming sample amount: 20 μL The parameters of mass spectrum ESI source by electrospray ionization Much reaction monitoring model M/Z: kaempferol, 285-93, dye lignin and 270-133 Detecting voltage: 3.0 KV Cone hole voltage: kaempferol, 42eV, collision can, 35eV Dye lignin and 30e, collision can, 32eV Capillary voltage: 32KV, ion source temperature: 110 ℃ Take off solvent transition temperature: 350 ℃ Take off 500l nitrogen flow: solvent relaxation/h Cone hole airflow: 50l/h 【results】 Rat stomach were determined after dosing filling within 24 hours kaempferol in rat body of different time blood drug concentration, painted blood drug concentration time curve, calculates the rat kaempferol major pharmacokinetic parameters, the main binders in plasma glucose hyaluronic acid ester form is enzymatic form. Determined to medicine rat stomach nanotubes filling the metabolism reckoned, phenol in urine as the prototype, the main form of glucose metabolism of hyaluronic acid ester enzyme binders and sulphuric acid ester enzyme combined forms, in bile out content, and mainly for very little in feces prototype, mainly in the form of prototype is excluded, the medicine in irrigation 50mg/kg, when in 25mg exclusion20 to/kg doses to5% from rate. 【conclusion】 Kaempferol in rats in vivo pharmacokinetic process conform to level the dynamics model, in plasma glucose hyaluronic acid ester enzyme mainly for combined, in urine in main glucose combined and hyaluronic acid ester enzyme sulfuric acid ester enzyme combined form existence, in the prototype in feces, much in from a few, expel the bile amount in vivo kaempferol reaches the peak time is short, metabolic slower, show medicine time cant time interval is too long, otherwise cant maintain effective blood drug concentration. 【key words