锌修饰的硅酸钙生物陶瓷涂层对mc3t3-e1细胞生物学影响的实验研究-experimental study on biological effects of zinc-modified calcium silicate bioceramic coating on mc 3t3 - e1 cells.docx

第二军医大学硕士学位论文 -  PAGE 4 - mineralization was observed by alizarin red staining. Results 1、XRD shows that the main of ingredients in. zinc-modified CaSiO3 bioceramics powders is Ca2ZnSi2O7 ,and other rare ingredients are Ca2SiO4 与 CaSiO3.There are no significant difference between the original zinc-modified CaSiO3 bioceramics powders and coating materials in component.SEM observe that the coating has a rough and uneven surface,and the particle size is about 60-120um. ICP results show that the material can release calcium ions(96.60±6.37ppm)、silicon ions (39.03 ± 6.29ppm) and zinc ions (9.05 ± 0.79ppm),and the material has a better chemical stability than CaSiO3 bioceramics coating. Cytotoxicity test showes that zinc-modified CaSiO3 bioceramics coatings are nontoxic and have good biocompatibility. 2、MC3T3-E1 cells culture in zinc-modified CaSiO3 bioceramics coatings, CaSiO3 bioceramics coatings and uncoated titanium.The cells attach more quickly on zinc-modified CaSiO3 bioceramics coatings(P＜0.05),and the early and late proliferation of cells also increases significantly on the coating(P＜0.05). The rate of adhesion and proliferation is higher than CaSiO3 bioceramics coatings,which indicate that the presence of zinc ions may benefit to adhesion and proliferation of cells and the coatings has a better cell compatibility. 3 、 The outcome of enzyme-linked immunosorbent assay and experiment of mineralization shows that the zinc-modified CaSiO3 bioceramics coatings can promote the secretion of alkaline phosphatase in the early stage,and can improve the synthesizing of type I collagen(P 0.05) and osteocalcin (P 0.05) in the early and late stage of MC3T3-E1cells differentiation.The coating can promote early osteoblast calcification,and have a better ability of differentiation and mineralization than CaSiO3 bioceramics. Conclusion Plasma Sprayed zinc-modified CaSiO3 Bioceramics Coating has excellent biocompatibility for MC3T3-E1 cells.It can b