响应面法优化链霉菌LG―9发酵条件及对棉花黄萎病菌抑菌作用.docVIP

响应面法优化链霉菌LG―9发酵条件及对棉花黄萎病菌抑菌作用 　　摘要：为提高链霉菌菌株LG-9发酵液的抑菌活性，在单因素试验的基础上，利用Minitab软件中的Plackett-Burman设计和响应面分析，以发酵液对棉花黄萎病菌（Verticillium dahliae）的抑菌圈大小为响应值，对链霉菌LG-9的液体发酵条件进行优化。结果表明，菌株LG-9的最佳发酵条件为250 mL三角瓶装样量101.19 mL、培养基初始pH 7.2、接种量7.5%、温度31.32 ℃、转速160 r/min和摇培时间69.02 h。在此条件下的抑菌圈直径大小为22.45 mm，较未优化前提高10.43%。 　　关键词：棉花黄萎病菌（Verticillium dahliae）；链霉菌；响应面分析；发酵条件优化 　　中图分类号：TQ920.1；S435.621 文?I标识码：A 文章编号：0439-8114（2018）08-0071-06 　　DOI：10.14088/j.cnki.issn0439-8114.2018.08.019 　　Optimization of Fermentation Conditions for Anti-streptomyces LG-9 Using Response Surface Methodology and Its Inhibiton on Verticillium dahliae 　　CHEN Ming1，MUKARAM Ablat1，LIU Zheng2，WANG Xiao-dong1 　　（1.College of Agronomy，Shihezi University/Key Laboratory of Oasis Crop Disease Prevention and Control，Shihezi 832000，Xinjiang，China； 　　2.Institute of Plant Protection，Shihezi Academy of Agricultural Sciences，Shihezi 832000，Xinjiang，China） 　　Abstract： In order to improve the antibacterial activity of Streptomyces isolate LG-9，on the basis of single factor test，using the Plackett-Burman experiment design and response surface analysis of Minitab software，with the inhibition circle size of the fermentation broth on Verticillium dahliae as the response value，and the liquid fermentation condition of Streptomyces LG-9 were optimized. The results showed that the optimal fermentation conditions of the strains LG-9 were loading volume of liquid medium 101.19 mL in 250 mL triangle bottle，pH 7.2，inoculation amount of 7.5%，temperature 31.32 ℃，rotate 160 r/min，time 69.02 h. The diameter of the inhibition zone under these conditions was 22.45 mm，which was 10.43% higher than that before optimization. 　　Key words： Verticillium dahliae； streptomyces； response surface analysis； optimization of fermentation conditions 　　棉花黄萎病是由大丽轮枝菌（Verticillium dahliae）引起的土传维管束病害，病原菌寄主范围广、危害重且缺乏有效的防治手段，被称为棉花的“癌症”。利用拮抗微生物来防治棉花黄萎病已经成为该领域的研究热点[1]。“以菌治菌”的生物防治对环境友好，无残留，作物不易产生抗药性，有些生防微生物还能促进农作物的生长。目前，防治棉花黄萎病报道最多的生防微生物有细菌[2，3]、真菌[4，5]和放线菌[6