沙葱AlliummongolicumRegel离体培养再生可育植株-植物研究.PDF

23 　 1 　　　　　　　　　　　　　　　　　　　　　　 2003　1 Vol.23　No.1　　　　　　　　　　　BULLETIN OF BOTANICAL RESEARCH Jan., 　2003 (A llium mongolicum Regel) 陈　刚 贾敬芬＊＊ 郝建国 (, 710069) 　　以沙葱(A l lium mongolicum Regel)的叶基切段作外植体, 成功地诱导出愈伤组织和再 生植株。诱导愈伤组织的培养基为附加2mgL-1 2, 4- 和0.2mgL-1 KT 的MS 培养基。愈伤 组织 移到附加2mgL-1 6BA 和0.4mgL-1 NAA 的MS 培养基后分化出大量的苗。分化苗在含 -1 有IBA 1mgL 的1/2MS 培养基上生根最佳。再生植株移栽成活率在95%以上, 移栽到田间的 植株第二年以后可正常开花结实。 　沙葱;组织培养;植株再生 FERTILE PLANT REGENERATION FROM TISSUE CULTURE IN AL LI UM M ON GOLI CUM REGEL ＊＊ CHEN Gang JIA Jing-Fen HAO Jian-Guo (Life Science School, Northwest University , Xian 710069) Abstract 　A highly efficient regeneration system of A l lium mongolicum Regel was established from -1 leaf base segments via tissue culture.MS medium supplemented with 2mgL 2, 4- and 0. -1 2mgL KT was suitable for callus induction.The shoots differentiated on MS medium added with -1 -1 -1 2mgL 6BA and 0.4mgL NAA.The half strength MS medium containing 1mgL IBA was more effective for the inducing roots.The regenerated plantlets were transplanted to the soil, and over 95%of them were survival.At the second year after transplanting, the plantlets flowered and set seeds. Key words　A l lium mongol icum Regel;tissue culture;plant regeneration 　　(A lli um mongo licum Regel) , 。 , 。 500 , [2 ～4] , (A .cep a L.) 、 (A .sat ivum