XRCC3和hOGG1与食管鳞状细胞癌放疗疗效及预后的相关性分析-肿瘤学专业论文.docx

新疆医科大学硕士学位论文 新疆医科大学硕士学位论文 PAGE PAGE 1 XRCC3和hOGG1与食管鳞状细胞癌放疗疗效及预后的 相关性分析 研究生：梁艳 导师：伊斯刊达尔·阿布力米提 摘 要 目的：探讨食管鳞状细胞癌(ESCC)癌组织中X射线修复交叉互补基因3(XRCC3) 和人类8-羟基鸟嘌呤糖苷酶1 (hOGG1)的蛋白表达与食管鳞癌放射治疗疗效及预后 的相关性。方法：采用免疫组织化学SP法检测了171例治疗前ESCC癌组织标本中 XRCC3和hOGG1的蛋白表达，结合临床病理特征和随访数据进行分析。结果：XRCC3 蛋白主要表达于细胞核，hOGG1蛋白主要表达于细胞核与线粒体，两者表达有相关 性。XRCC3蛋白在无饮酒史的阳性表达率和有饮酒史的阳性表达率分别为73.5％和 54.2％，其差异具有统计学意义(P＝0.046)。hOGG1蛋白在病变长度≤5cm和病变长 度＞5cm 的阳性表达率分别为81.7％和66.7％，其差异具有统计学意义(P＝0.022)。 且hOGG1蛋白在中胸段、下胸段和颈段、上胸段病变部位的阳性表达率为79.5％和 64.8％，其差异具有统计学意义(P＝0.040)。hOGG1蛋白在原发灶分期(T1、T2)和原 发灶分期(T3、T4)的阳性率分别为82.5％和64.9％，差异具有统计学意义(P＝0.009)。 XRCC3表达阳性组中位生存期与表达阴性组中位生存期分别为54月和49月，其差异 无统计学意义(P＝0.683)。hOGG1表达阳性组的中位生存期和表达阴性组的中位生存 期分别为54月和49月，其差异无统计学意义(P＝0.780)。多因素分析结果显示疗效评 价、病变长度与食管癌的预后相关(P分别为0.005和0.045)。结论：XRCC3、hOGG1 的蛋白表达情况可能在食管癌的发生中起一定作用。hOGG1的蛋白表达与食管癌的 病变部位、病变长度及原发灶分期密切相关，XRCC3、hOGG1的蛋白表达情况与预 后无相关。 关键词：食管癌；放射治疗；XRCC3；hOGG1；预后 A Correlation Study of XRCC3 and hOGG1to the Effect of Radiotherapy and Prognosis in Esophageal Squamous Cell Carcinoma Postgraduate: LIANG Yan Supervisor: Professor YISIKANDAER·Abulimiti Abstract Objective: To analyze the relationship between the protein expressions of X-ray repair cross-complementing group 3, human 8-oxoguanine DNA glycosylase 1 and the effect of radiotherapy and prognosis in esophageal squamous cell carcinoma (ESCC). Methods: The protein expression of XRCC3 and hOGG1 in 171 samples of ESCC tissues were examined using SP immunohistochemical staining and were analyzed according to the clinic pathologic features and follow-up data. Results: XRCC3 protein were expressed in the nucleus of tumor cells in ESCC. And hOGG1 protein were expressed in the nucleus and mitochondria of tumor cells in ESCC. The positive expression rate of XRCC3 protein in the drunk group and non-drunk group were 73.5％ and 54.2％, respectively (P＝0.046). The positive expression rate of hOGG1 protein in the group of length just and more than 5 cm and the group of length less than 5 cm a were 81.7％ and 66.7％, respectivel