口腔扁平苔藓患者外周血microRNA-155及microRNA-146a的表达.DOCVIP

慢性牙周炎患者唾液中miR-146a的表达及其与临床牙周参数的相关性研究 赵家珍，闫文娟，吴补领 通信作者：吴补领 联系电话：020基金项目] 国家自然科学基金面上项目（南方医科大学南方医院牙体牙髓病— 通信作者：吴补领 联系电话：020[基金项目] 国家自然科学基金面上项目 【摘要】 目的 通过检测慢性牙周炎患者唾液中miR-146a的表达，以探讨miR-146a在慢性牙周炎发病中的作用及意义。方法 收集慢性牙周炎患者48例，男性28例，女性20例，年龄 27～58岁。同时选择20名性别、年龄匹配的健康志愿者作为对照组。采用实时荧光定量PCR技术检测上述人员唾液上清中miR-146a的表达水平。对治疗组患者（32例）进行牙周基础治疗，分别记录治疗前和治疗6周后患者牙周临床指数（包括探诊深度、附着丧失及菌斑指数），并检测治疗后唾液上清液标本中miR-146a的表达。结果 慢性牙周炎患者唾液上清液中miR-146a的表达水平(2.52±0.87)显著高于健康对照组(0.81±0.58) (P＜0.01)。治疗组患者经过治疗后的牙周临床指数、唾液中miR-146a表达水平均显著降低（P＜0.05)。治疗前后miR-146a的表达变化与探诊深度、附着丧失及菌斑指数均呈正相关(P＜0.01)。结论 慢性牙周炎患者唾液上清中miR-146a表达水平显著升高，并在牙周基础治疗后显著下降，提示唾液miR-146与牙周炎症程度相关，唾液miR-146a可作为牙周病防治及疗效评估的潜在靶分子。 【关键词】慢性牙周炎；唾液；微小RNA；miR-146a Expression of MicroRNA-146a in saliva in patients with chronic periodontitis and its correlation study of clinical periodontal parameters Zhao Jiazhen, Yan Wenjuan,Wu Buling. (Dept.of Endodontics ,Nanfang Hospital of Southern Medical University，National clinical key specialty ；College of Stomatology，Southern Medical University，Guagnzhou 510515，China) 【Abstract】 Objective To investigate the expression of miR-146a in saliva from patients with chronic periodontitis and their significance, and clarify its role in the development of chronic periodontitis. Methods Twenty-eight male and Twenty female chronic periodontitis patients ages 27 to 58 years were selected. All chronic periodontitis patients were newly-diagnosed and treatment-free. Twenty healthy volunteers matching in sex and age were also enrolled as controls. miR-146a expression in saliva was examined by Real-time PCR. 32 chronic periodontitis patients were treated with initial therapy for 6 weeks, Fresh saliva samples were collected before and after the treatment, and clinical indices were recorded. The expression of miR-146a in saliva was also assessed by Real-time PCR. Results The expressions of miR-146a in saliva was higher in chronic periodontitis patients (2.52±0.87) than those in the healthy co