基于电化学阻抗谱EIS的生物细胞检测方法.PDF

第 4 卷 第 2 期 生 物 化 工 Vol.4 No.2 2018 年 4 月 Biological Chemical Engineering Apr. 2018 文章编号：2096-0387（2018）02-0004-05 基于电化学阻抗谱（EIS）的生物细胞检测方法 1 1 1 2 1 1 姚佳烽 ，姜祝鹏 ，徐梓菲 ，刘夏移 ，陈柏 ，吴洪涛 （1. 南京航空航天大学 机电学院，江苏南京 210000；2. 西安理工大学 机械与精密仪器学院，陕西西安 710000） 本文利用电化学阻抗谱（EIS）对酵母菌细胞的介电性能进行了探究，通过实验，测量了酵母菌细胞悬浮液的电容， 摘　要： 并计算出相对介电常数和介电损耗。结果显示，随着酵母菌细胞浓度的增加，相对介电常数和介电损耗也增加。此外，实验结果 还显示活细胞的相对介电常数和介电损耗高于死细胞。本研究证明，EIS 方法可用来检测细胞悬浮液的状态。 电介质；电化学阻抗谱；酵母菌；细胞检测 关键词： 中图分类号：O657.1　　　　文献标志码：A Measurement of Biological Cells by Electrical Impedance Spectroscopy 1 1 1 2 1 1 Yao Jia-feng ，Jiang Zhu-peng ，Xu Zi-fei ，Liu Xia-yi ，Chen Bai ，Wu Hong-tao (1.College of Electrical and Mechanical Engineering, Nanjing University of Aeronautics and Astronautics, Jiangsu Nanjing 210000; 2.Institute of Machinery and Precision Instrument, Xian University of Technology, Shaanxi Xian 710000) Abstract: Impedance spectroscopy has been proved to be a useful technique for analyzing heterogeneous systems, especially for biological cell suspensions and tissues because of its capacity of non-invasive measurement. Experiments were conducted to study the dielectric properties of yeast cells by Impedance Spectroscopy. The capacitance of the yeast cell suspension was measured, the relative permittivity and the dielectric loss were calculated. As the concentration of yeast cells increased, the relative permittivity and the dielectric loss were also increased. In addition, the relative permittivity and the dielectric loss of living cells were higher than that of the dead ones. Finally, we concluded that it is possible to sense the status and the concentration