【毕业论文】不同产地款冬花中总黄酮的含量测定.docVIP

泰山医学院本科毕业设计（论文） 学号:4107013035 毕业设计（论文） 题目：不同产地款冬花中总黄酮的含量测定 院（部）系 药学院 所 学 专 业 年级、班级 完成人姓名 指导教师姓名 专业技术职称 2011年 6 月 17 日 摘 要 目的 款冬花中黄酮类化合物的生物活性较高，本实验通过对黄酮类成分几种不同提取方法的对比，确定最优提取方法。用最优提取方法分别测定15种不同产地的款冬花中总黄酮成分含量，为款冬花药材质量控制提供实验依据。 方法 本实验用于款冬花中总黄酮成分的提取方法主要有以下几种：索氏提取法、醇提回流提取法以及超声提取法。其中，索氏提取法的工艺条件为：取款冬花粗品1.0421g，加入190ml 50%乙醇，提取温度为85℃，提取时间为6h；醇提法工艺条件为：醇提取量为款冬花的30倍分两次回流提取，提取温度为85℃，两次的提取时间共3.5h；超声提取法采用2.0201g荷叶粉末，30ml 50%的乙醇在25℃下超声提取30min。含量测定方法采用紫外可见分光光度法，以芦丁为对照品，样品乙醇提取后，用亚硝酸钠，硝酸铝，氢氧化钠显色后测定含量。 结果 醇提回流提取法提取最为完全。用醇提回流提取法分别对15种不同产地的款冬花进行提取，用紫外分光光度法测得的吸光度各不相同。 结论 不同产地的款冬花中总黄酮成分含量不同。 关键词：款冬花；总黄酮；提取方法；紫外分光光度法；含量测定 Abstract Objective: KuanDong in the flower of flavonoid compounds biological activity is higher, the experiment of flavonoids ingredients through several different extraction method contrast, determine the optimum extraction method. With the optimum extraction method were determined 15 different origin of flowers KuanDong flavonoid content for KuanDong flowers, herbs provide the basis for quality control. Methods: This experiment used to KuanDong in the flower of flavonoid extract method basically has the following kinds: soxhlet extraction, ethanol-extraction reflux extraction and the ultrasonic extraction. Among them, saxhlet extraction technology conditions for: a withdrawal winter spend crude products, to join 190 ml 1.0421 g without water, ethanol extraction temperature for 85 ℃, extracting time for 6 h; Alcohol formulation process conditions for: alcohol extraction KuanDong flowers is 30 times two points reflux extraction, extraction temperature for 85 ℃, two extracting time of 3.5 h; The ultrasonic extraction using 2.0201 g lotus leaf powder, 30 ml 50% ethanol in 25 ℃ ultrasonic extraction under 30 min. The content determination methods using ultraviolet spectrophotometry, rutin ethanol-water, ethanol, with samples after nitric acid sodium nitrite, aluminum, sodium hydroxide show color determ