（毕业设计论文）《正交实验提取生姜黄酮和姜辣素的工艺研究》.doc

摘 要 以生姜为原料，研究了超声波法提取生姜黄酮和姜辣素的工艺条件。在单因素实验的基础上，选取乙醇浓度、超声时间、超声温度、物料比为影响因素，通过正交实验研究提取的最佳工艺条件。实验结果表明：以65%乙醇溶液为提取溶剂，提取时间25min，提取温度50℃，物料比1：30，超声波功率140W，提取姜黄酮效果最佳，此条件下的生姜黄酮得率可达1.0550%。以乙醇浓度65%，提取时间25min，提取温度60℃，物料比1：30，超声波功率140W，提取姜辣素效果最佳，此条件下的姜辣素提取率为7.8776mg/g。 关键词：超声波法提取；生姜；姜黄酮；姜辣素；正交实验 ABSTRACT With ginger as the raw material, this paper reaches into the processing condition of Ultrasonic Extraction of ginger flavone and gingerol. Based on the singer factor experiment, it selects ethanol concentration, ultrasound time, ultrasound temperature, material ratio as the influence factor to study and extract the best processing condition by the experiment of orthogonality. The experiment shows that, 65% ethanol liquor as extract dissolvent, 25 min as extract time, 50℃ as extract temperature, 1:30 as material ratio, 140 W as ultrasonic power are the best processing condition to extract ginger flavone and the ratio to extract ginger flavone can be 1.0550%. And it is the best condition to extract gingerol and the ratio is 7.8776. Key words: Ultrasonic Extraction；ginger；ginger flavone；gingerol；orthogonality 目 录 TOC \o 1-4 \h \z \u HYPERLINK \l _Toc295480404 摘 要 = 1 \* ROMAN I HYPERLINK \l _Toc295480405 ABSTRACT = 2 \* ROMAN II HYPERLINK \l _Toc295480406 第一章 绪 论 1 HYPERLINK \l _Toc295480407 1.1姜黄酮和姜辣素的性能简介 1 HYPERLINK \l _Toc295480408 1.2 姜黄酮和姜辣素提取方法的研究现状 1 HYPERLINK \l _Toc295480409 1.3超声波提取原理简介 1 HYPERLINK \l _Toc295480410 1.4超声波提取法的应用 2 HYPERLINK \l _Toc295480411 1.4.1采用超声波技术提取黄酮 2 HYPERLINK \l _Toc295480412 1.4.2采用超声波技术提取姜辣素 2 HYPERLINK \l _Toc295480413 1.5超声波法讨论因素分析 2 HYPERLINK \l _Toc295480414 第二章 实验内容 4 HYPERLINK \l _Toc295480415 2.1 实验药品 4 HYPERLINK \l _Toc295480416 2.1.1 实验所需主要溶液的配制 4 HYPERLINK \l _Toc295480417 2.3 实验方法 5 HYPERLINK \l _Toc295480418 2.3.1 总黄酮含量测定 5 HYPERLINK \l _Toc295480419 2.3.2 姜辣素含量测定 6 HYPERLINK \l _Toc295480420 2.3.3 工艺优化 6 HYPERLINK \l _Toc295480421 提取溶剂选择 6 HYPERLINK \l _Toc295480422 乙醇浓度的选择 7 HYPERLINK \l _Toc295480