（毕业设计论文）《八角提取物对蚜虫生物活性及对其解毒酶活力的影响》.docVIP

精品 安 徽 农 业 大 学 毕 业 论 文（设计） 论文题目 八角茴香提取物对蚜虫的生物活性及其解毒酶活性的影响 姓 名 学 号 学 院 植保学院 专 业 植 物 保 护 指导教师 职 称 副 教 授 中国·合肥 二o一二年 六 月 目 录 摘要·······························1 引言··································1 1材料与方法································2 1.1试验材料·························································2 1.2供试昆虫·························································2 1.3试剂与仪器·······················································2 1.4 八角茴香果实有机溶剂提取物的制备································2 1.5 试验方法·························································3 1.5.1生物活性测定···················································3 1.5.2蛋白质测定·····················································3 1.5.3乙酰胆碱酯酶活性测定···········································3 1.5.4谷胱甘肽转移酶活性测定·········································3 2结果与分析························································4 2.1 不同有机溶剂对八角茴香干果的提取率······························4 2.2不同剂量的2种溶剂八角茴香提取物的触杀作用·······················5 2.2 甲醇和乙酸乙酯不同浓度溶剂提取物对蚜虫乙酰胆碱酯酶活力的影响······································································5 2.3甲醇和乙酸乙酯不同浓度溶剂提取物对蚜虫谷胱甘肽转移酶酶活力的影响·····································································6 3 小结与讨论·······················································7 致谢·······························································7 参考文献···························································8 英文摘要···························································9 精品 八角茴香提取物对蚜虫的生物活性及其解毒酶活性的影响 学生： 周翔 （安徽农业大学植物保护学院2008级动植物检疫专业，合肥230036） 指导老师：李世广 （安徽农业大学植物保护学院，合肥230036） 摘 要：使用甲醇、乙酸乙酯两种有机溶剂对八角茴香干果进行浸泡以提取杀虫活性物质。用丙酮对八角茴香提取物进行稀释，用八角茴香提取物对桃蚜进行试验，以明确不同有机溶剂提取液对蚜虫生物活性及其解毒酶活性的影响。结果表明：触杀试验中，不同浓度下2种提取物的杀虫效果相近，甲醇提取物整体杀虫效果比乙酸乙酯提取物好。成虫组织酶液的乙酰胆碱酯酶活力测定试验表明甲醇、乙酸乙酯提取物对成虫乙酰胆碱酯酶活力有明显影响。成虫组织酶液的酶液谷胱甘肽S－转移酶活力测定试验表明甲醇、乙酸乙酯提取物对成虫的谷胱甘肽S－转移酶活力有明显的抑制作用，浓度越高，抑制作用越大。 关键词：八角茴香；提取物；桃蚜；生物活性；乙酰胆碱酯酶；谷胱甘肽转移酶 八角茴香（Illicium verum Hook.f.），是木兰科