（毕业设计论文）便携式实时共聚焦显微镜的研制.doc

便携式实时共聚焦显微镜的研制 PAGE 精品 SHANGHAI JIAO TONG UNIVERSITY 学士学位论文 THESIS OF BACHELOR 论文题目：便携式实时共聚焦显微镜的研制 学生姓名: 学生学号: 专 业: 生物医学工程 指导教师: 学院(系): 生物医学工程 便携式实时共聚焦显微镜的研制 摘要 共焦显微镜能无创地对厚的透明和半透明物质内的薄截面成高分辨率、高对比度的像 [1]。采用共焦光学技术可观察组织表面200微米深的细胞精细结构与生物化学成份，因而非常适合于皮肤疾病的病理诊断。我们搭建了一套用于人体皮肤的反射式实时激光共聚焦显微镜。整个系统包括光学部分模块，扫描部分模块，同步电路和计算机处理模块及接口。利用一个多面镜和检流计，可以对1.4mm x1.4mm的区域以10帧/s成1000 x1000的像。利用一对侧向的步进电机，我们使视野扩展到7mm x7mm。利用z轴的步进电机，轴向扫描分辨率可以达到3微米。NI公司的图像采集卡PCI-1410可以对雪崩式光电二极管（APD）获得的图像信息和同步电路产生的水平和垂直同步信号进行处理，输出视频信号。整套系统采用LabVIEW软件进行控制。 关键词：共聚焦显微镜，多面镜，步进电机，PCI-1410，LabVIEW  CONSTRUCTION OF A PORTABLE CONFOCAL MICROSCOPE FOR IMAGING HUMAN TISSUE IN VIVO ABSTRACT A confocal scanning microscope is capable of noninvasively imaging thin sections within a thick transparent or translucent object with high resolution and contrast. The use of confocal optical technology can observe the fine structure and biochemical composition of cells 200-micron deep from the surface, thus it is very suitable for pathological diagnosis of skin diseases. We have built a real time confocal scanning microscope for reflectance imaging of human skin. The whole system includes the optical module, scanning modules, synchronization circuit, computer processing and the interface modules. A polygon mirror is utilized together with a galvanometer to realize a 1000x1000 image at a frame rate of 10 fps, covering a scan field of 1.4mm x1.4mm. A pair of lateral stepping motors are employed which extends the filed-of-view to 7mm x 7mm. By using z-stepping motors, axial scan is realized with the resolution of 3 microns. NI image acquisition card PCI-1410 can process the image information detected by the anavalanche photodiode (APD) and the horizontal and vertical sync signals generated by synchronization circuit, then output video signal. The entire system is controlled using LabVIEW software. Ke