针根中Mn和Al也较高,Mn为正常骨结构所必.PDF

近红外技术应用于药厂丹参药材质量检测 白雁，李磊，雷敬卫* ，谢彩侠，左春芳 (河南中医学院，郑州450008) 摘要：目的 利用近红外光谱技术，快速测定丹参药材中丹参酮ⅡA 的含量。方法 运用偏最小二乘法(PLS)建立丹参药 材中丹参酮ⅡA 含量与其近红外光谱之间的校正模型，并对未知的丹参样品进行丹参酮ⅡA 含量预测。结果 校正集内 部交叉验证决定系数R2 为0.996 94 ，交互验证均方根偏差RMSECV 为0.011 8 ，11 份样品的外部预测均方根偏差RMSEP 为0.023 7 。预测值的平均相对误差为1.712 9%。结论 近红外光谱技术测定丹参药材中丹参酮ⅡA 的含量是可行的，该 方法快速准确、简单无损，可应用于大批量样品的快速分析。 关键词：近红外光谱技术；丹参提取物；丹参酮ⅡA ；快速测定 中图分类号：R284.1 文献标志码：A 文章编号：1007-7693(2012)03-0222-04 基金项目：河南省重大公益科研项目(081100912500) ；河南中医学院2011 年博士科研基金(BSJJ2011-03) 作者简介：白雁，女，教授，博导 Tel:E-mail: 270938736@ *通信作者：雷敬卫，副教授，博士 Tel: (0371 E-mail: ljwei@ ·222 · Chin JMAP, 2012 March, Vol.29 No.3 中国现代应用药学2012 年3 月第29 卷第3 期 Quality Determination of Salvia Miltiorrhiza in Pharmaceutical Industry By Near-infrared Spectroscopy * BAI Yan, LI Lei, LEI Jingwei , XIE Caixia, ZUO Chunfang,(Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China) ABSTRACT: OBJECTIVE To rapidly determine the content of tanshinone ⅡA in Salvia miltiorrhiza extraction by near-infrared spectroscopy. METHODS The calibration model of the content of tanshinone ⅡA in Salvia miltiorrhiza and the near infrared spectroscopy of Salvia miltiorrhiza was built with partial least squares. RESULTS The correlation coefficient of 2 calibration (R ) was 0.996 94. Root mean square error of cross validation (RMSECV) was 0.011 8, and external prediction deviation RMSEP of 11 samples was 0.023 7. Predicted average relative error was 1.712 9%. CONCLUSION It is feasible to determine the tanshinone ⅡA content in Salvia miltiorrh. Near-infrared spectroscopy is fast, accurate, simple and nondestructive which can be applied in rapid analysis of large numbers of samples. KEY WORDS: near-infrared spectroscopy; Salvia miltiorrh; Tanshinone ⅡA; rapid determination 丹参为唇形科植物丹参(Salvia miltiorrhiza 1.2 材料