猪肝中维生素B12的检测及提取研究.doc

PAGE 2 猪肝中维生素B12的检测和提取研究 摘 要 目的：维生索B12广泛应用于医药、食品和畜牧业，工业化生产维生綮B12主要通过微生物发酵。动物源食品富古维生索B12，如内脏、肝、肾、牛乳及蛋黄。通过对猪肝中维生素B12的检测，了解到猪肝中维生素B12的量，从而可以清楚的通过摄取猪肝的量来维持人体内维生素B12的量。 方法： 1．原子吸收法测定维生素B12的量： 维生素B12分子中含有钴原子，采用原子吸收分光光度计可以测定维生素B12中的钴含量，再通过质量换算即可得到维生素B12的含量。 2．大孔树脂吸附法（CAD45）提取维生素样品中的B12 ： 张玉明的研究发现，CAD45树脂在pH为7时对羟基钴胺素具有良好的吸附能力，并且使用50％乙醇(pH=2)溶液可以作为良好的洗脱剂，静态洗脱率高达99％以上。 3. 使用高效液相色谱法检测其中羟基钴胺素的量： 用HPLC，采用乙腈和醋酸钠缓冲液为流动相能快速测定羟基钴胺素的量，从而确定维生素B12 的提取率。 关键词：维生索B12 ；羟基钴胺素；大孔树脂吸附法；原子吸收法；高效液相色谱法 Detection and extraction of the liver in vitamin B12 Abstract Objective: Vit B12 is widely used in medicine, food and livestock, industrial production of subsistence embroidered banner B12 mainly through microbial fermentation. Food of animal origin, rich in ancient Vit B12, such as offal, liver, kidney, milk and egg yolk. Understand the amount of vitamin B12 in the liver in the detection of vitamin B12 in the liver in, which can clearly maintain the human body the amount of vitamin B12 uptake of the liver volume. Methods: 1.The atomic absorption method for the determination of the amount of vitamin B12: Vitamin B12 molecules containing cobalt atoms by atomic absorption spectrophotometer can determine the content of cobalt in vitamin B12, vitamin B12 can be obtained through quality conversion. 2. Macroporous resin adsorption (CAD45) extract vitamin samples B12: The Yuming the studies have found that The CAD45 resin at pH 7:00 hydroxy-cobalamin prime has a good adsorption capacity and use 50% ethanol (pH = 2) solution can be used as the eluent, static elution rate of 99% or more . 3.Using high performance liquid chromatography detection of hydroxy-cobalamin amount: By HPLC, using acetonitrile and sodium acetate buffer as the mobile phase to rapid determination of the amount of hydroxy-cobalamin in order to determine the extraction rate of vitamin B12. Keywords: Vit B12 and; hydroxy cobalamin; macroporous resin adsorption; atomic absorption spectrometry; high performance liquid chromatography. 目