有哪些信誉好的足球投注网站- 1、本文档共7页，可阅读全部内容。
- 2、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
- 3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
查看更多
利用16SrRNA基因克隆文库技术分析德昌水牛瘤胃产甲烷菌的多样性
() 2014 8 40 4
Journal of Hunan Agricultural University (Natural Sciences), Aug. 2014, 40(4):382–388
DOI:10.13331/ki.jhau.2014.04.011
/qks
利用 16S rRNA 基因克隆文库技术分析
德昌水牛瘤胃产甲烷菌的多样性
1,2 1,2 1,2 1,2* 1,2
(1. 530001 2.() 530001)
摘 要3 5 DNA
Met86F/Met1340R 16S rRNA 16S rRNA
99 16S rRNA RDP 94.2%
(Methanobrevibacter)16S rRNA 97%19 96 (17 OTUs)
97.0%16S rRNA 97% 3 (2 OTUs)3.0%
16S rRNA 90%~97% SGMT RO
75.8%1.0% Methanobrevibacter
Methanobrevibacter Methanobrevibacter
关 键 词16S rRNA
+
中图分类号S823.8 3 S852.6 文献标志码：A 文章编号：1007− 1032(2014)04−0382−07
16S rRNA library-based analysis of ruminal methanogens diversity
in Dechang water buffalo
YANG Cheng-jian1,2WEI Sheng-ju1,2LIANG Xin1,2LIANG Xian-wei1,2*ZOU Cai-xia1,2
(1.Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China; 2.Key
Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Ministry of Agriculture, Naning 530001, China)
Abstract: Three femal Dechang water buffaloes were used in this study. Total DNA were extracted by bead-beating method.
Primers of Met86F/Met1340R were used to amplify the 16S rRNA of archaea for the construction of library. Results showed
that 99 sequences were obtained. RDP analysis showed that 94.2% sequences are classified to genus Methanobrevibacter .
Based the 97% similarity, these sequences were assigned to 19 OTUs. Ninety-six sequences showed ≥97% sequence
similarity to known species, three
文档评论（0）