亚麻木酚素对糖尿病小鼠肾脏保护的作用及机制.docVIP

亚麻木酚素对糖尿病小鼠肾脏保护的作用及机制 　　(1.淮安市第二人民医院内分泌科,江苏 淮安 223002; 　　2.南京医科大学第二附属医院营养科,江苏 南京 210011; 　　3.江苏省疾病预防控制中心,江苏 南京 210009) 　　摘 要:目的:观察亚麻木酚素提取物(FLE)对糖尿病小鼠早期肾损伤的影响并探讨其作用机制。方法:雄性ICR小鼠分6组:正常对照组,糖尿病模型对照组,糖尿病模型亚麻木酚素提取物[600mg/(kg#8226;d)、1.2g/(kg#8226;d)、3.6g/(kg#8226;d)]治疗组,糖尿病小鼠盐酸二甲双胍[100mg/( kg#8226;d)]治疗组。连续灌胃8周,实验结束后比较各组体重、肾重、肾重/体重、24h尿白蛋白排泄率、肾组织MDA含量及肾组织SOD、GSH-PX活性,HE染色分析肾小球病理形态学改变,综合评价亚麻木酚素提取物对肾脏的保护作用。结果:与糖尿病模型对照组比较,亚麻木酚素提取物中、高剂量组的肾重、24h尿白蛋白排泄率显著降低(P0.05),MDA含量降低(P0.05),亚麻木酚素提取物各剂量组的肾脏组织SOD、GSH-Px活性增加(P0.05)。结论:亚麻木酚素提取物能减轻糖尿病小鼠肾脏损伤,对肾脏有保护作用,其机制与其减轻肾脏氧化应激有关。 　　关键词:木酚素;糖尿病;肾脏;氧化应激? 　　中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)04-0856-04 　　Study on the Protective Effect of Flaxseed Lignan Extract on Kidney in Diabetic Mice 　　HONG Bing1, XU Hai?e2, HU Wen1, LI Li3, XU Xiao?wen1,XU Wei1, MA Shao?gang1,YU Wei?nan1 　　(1.The department of endocrinology, the Second Peoples Hospital of Huaian, Huaian 223002, Jiangsu,China; 　　2.The department of nutrition, the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210011,Jiangsu,China; 　　3.Jiangsu Provincial Center for Disease Control and Prevention,Nanjing210009, Jiangsu,China)? 　　Abstract:Objective: To investigate the protective effect of of Flaxseed Lignan Extract(FLE)on kidney in diaetic mice. Methods: ICR mice were randomly divided into 6 groups: normal mice control, STZ diabetic mice control, STZ diabetic mice fed with FLE at dose of [600mg/(kg#8226;d),1.2g/(kg#8226;d), 3.6g/(kg#8226;d)], and STZ diabetic mice fed with metformin. The above components were administed intragastrically for 8 weeks. Then their blood glucose, kidney weight to body weight, 24-hour urinary protein,the content of MDA and the activities of SOD, GSH-Px of kidney were detected. Glomerular structure of kidney were examined by light microscopy. Results:The kidney weight to body weight, 24-hour urinary protein in high,middle dose of FLE groups were significantly lower than those in diabetic control group(P0.05). The content of MDA in