B细胞线性表为的精细定位15p.pptxVIP

Fine mapping and conservation analysis of linear B-cell epitopes of peste des petits ruminants virus nucleoprotein;ABSTRACT:;Peste des petits ruminants virus (PPRV), a Morbillivirus, causes an acute, highly contagious disease of domestic and wild small ruminants characterized by respiratory and gastrointestinal pathology (Munir, 2013). PPRVs have been classified into four lineages based on variable nucleotide sequences of the nucleoprotein (NP) gene. Western and Central African PPRVs cluster into Lineages I and II, Eastern African and PPRVs found in the southern part of the Middle East cluster into Lineage III, while Asian PPRVs cluster mainly into Lineage IV (Munir et al., 2013). Among the six structural proteins of morbilliviruses, the NP is the most abundant and highly immunogenic in spite of its internal location, and forms the basis of most diagnostic assays for PPRV (Libeau et al., 1995).Growing interest in the diagnostic applications of PPRV NP has focused attention on identifying more antigenic epitopes. B cell epitopes (BCEs) are categorized either as linear (composed of continuous aa) or conformational (discontinuous aa).. In nature, the majority of BCEs are discontinuous but, due to difficulties in the mapping and design of such epitopes, more research has centered on linear BCEs (Han et al., 2013). ;Epitope mapping on PPRV NP has been reported using monoclonal antibodies/deletion mutants and com_x0002_puter based prediction algorithms/indirect ELISA (Choi et al., 2005b; Dechamma et al., 2006). Hitherto, however,there are no reports of in-depth mapping and minimal motif identification of BCEs associated with PPRV NP. An efficient method of epitope screening, which has been adopted for fine mapping of epitopes on SARS S protein (Hua et al., 2005), involves the use of biosynthetic eptides (Paterson et al., 1998; Xu et al., 2012). This strategy has now been used to identify 19 linear epitopes and their minimal motifs on the NP of PPRV. Data from this resear