四逆散对高脂血症小鼠血脂代谢途径的影响-环球中医药杂志.PDF

1摇450 环球中医药2016年12月第9卷第12期摇 Global Traditional Chinese Medicine,December2016,Vol郾9,No郾12 ·论著 · 四逆散对高脂血症小鼠血脂代谢途径的 影响 吴越摇 朱峰摇 刘建平摇 李永民 揖摘要铱摇 目的摇 探讨四逆散对高脂血症C57BL/ 6小鼠脂代谢的影响并初步探讨其机制。 方法 摇 40 只SPF级 C57BL/ 6 小鼠作为研究对象,随机分为对照组、模型组、四逆散(0.9 mg/ g体质量) 组、枳实(0.9 mg/ g体质量)组,分别以生理盐水、四逆散水煎剂、枳实水煎剂灌胃,干预时间为90 天。 全自动生化分析仪检测血清甘油三脂(triglyceride,TG),低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL鄄C),高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL鄄C)、 ELISA法检测血清载脂蛋白A鄄1(apolipoprotein A鄄1,APOA鄄玉)水平及实时定量反转录-聚合酶链反 应检测肝脏以及小肠组织中APOA鄄I 的mRNA基因表达情况。 结果摇 在脂代谢方面,四逆散干预可 显著防止高脂饮食小鼠血清TG、LDL鄄C水平的升高(P0.05),显著提升血清中HDL鄄C和APOA鄄I水 平(P0.05),显著增加肝脏中APOA鄄I 的mRNA表达程度(P0.05)。 结论摇 四逆散可能是通过促进 肝脏APOA鄄I mRNA表达和增加APOA鄄I分泌来干预血脂水平。 揖关键词铱摇 四逆散;摇 高脂血症;摇 载脂蛋白A鄄I 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2016郾12郾003 Effects of Sini powder on blood lipid metabolism in mice with hyperlipidemia摇 WUYue,ZHUFeng, LIUJian鄄Ping,et al.摇 Shanxi University of Traditional Chinese Medicine,Taiyuan030024,China Corresponding author:LI Yong鄄min,E鄄mail:liyongmin2001@ 揖Abstract铱摇 Objective 摇 To observe the effects of Sini powder on C57BL/ 6J mice with hyperlipidemia and exploreitsmechanism. Methods摇 40 C57BL/ 6 micewererandomly dividedintoblank control group,hyperlipidemia group,hyperlipidemia plusSini powder (0郾9 mg/ gweight) group and hyper鄄 lipidemia plusfructus aurantii immaturus (0.9mg/ g weight). Blank control group were fed with normal diet and the other groupswerefedwith highfat diet. Micewere received gavage administrationwith corre鄄 sponding drug,Sini powder andfructusaurantii immaturuswere intervenedfor90 days. The automaticbio鄄 chemistry analyzers methodwasusedto measurethe levels of TG,LDL鄄C,HDL鄄Cin serum. Elisa methods were used to detect the APOA鄄I levels in serum. Real鄄time PCR methodswere used to detect the APOA鄄I mRNA expression chang