虫生真菌分子致病机理和基因工程改造研究进展.PDF

微生物学通报 MAR 20, 2008, 35(3): 443~449 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 专论与综述 虫生真菌分子致病机理及基因工程改造研究进展 1,2 1 2* 吕丁丁 李增智 王成树 (1. 230036) (2. 200032) 摘 要: 虫生真菌侵染寄主昆虫的复杂过程可分为体表附着、体壁穿透及体内定殖和致死等不同 阶段。近年来, 以金龟子绿僵菌(Metarhizium anisopliae)和球孢白僵菌(Beauveria bassiana)为代表 的基因功能研究取得了长足的进展, 从不同角度阐明了虫生真菌的分子致病机理; 同时, 基因工 程技术的应用为昆虫病原真菌的遗传改良和选育高毒力杀虫菌株开辟了新的途径。对近年来昆虫 病原真菌侵染寄主的分子对策及基因工程改造的研究进展进行了综述, 并就进一步研究虫生真菌 的毒力基因及功能进行了探讨。 关键词: 虫生真菌, 致病机理, 毒力基因, 基因工程 Advances in Molecular Pathogenesis and Genetic Engineering of Entomopathogenic Fungi LV Ding-Ding1,2 LI Zeng-Zhi1 WANG Cheng-Shu2* (1. Anhui Provincial Key Laboratory of Microbial Control , Anhui Agricultural University , Hefei 230036) (2. Institute of Plant Physiology Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032) Abstract: The complex of insect mycoses by entomopathogenic fungi includes, in general, spore adhering, cuticle penetration, hemocoel colonization and host death. Recent gene function studies of Metarhizium ani- sopliae and Beauveria bassiana have bettered the understanding of the mechanisms of molecular entomopa- thogenicity that there are different genes involved in different infection stages. With the identifications of different virulent genes and the exploring of exogenous toxins, genetic