代谢组学在肿瘤研究中的应用课件.ppt

p53 regulation of energy metabolism 2-1 p53 in tumor cell metabolism 2-1 p53 in tumor cell metabolism: glutamine metabolism Biochemistry of glutamine metabolism (main pathways): glutaminase 2-1 p53 in tumor cell metabolism: glutamine metabolism 2-1 p53 in tumor cell metabolism: glutamine metabolism Model for regulation of intracellular ROS levels by GLS2. 2. 2 HIF-1 与肿瘤代谢 2-2 HIF-1 in tumor cell metabolism HIF-1: upstream and downstream of cancer metabolism. Curr Opin Genet Dev. 2010 Feb;20(1):51-6. 2-2 HIF-1 in tumor cell metabolism HIF-1: activating transcription of genes encoding glucose transporters and glycolytic enzyme Expression of genes encoding glucose transporters and glycolytic enzymes. The glycolytic pathway is shown at left. Symbols for genes encoding the respective enzymes are coded by font according to the mRNA expression pattern (normalized to 18S rRNA) in ES cells cultured under nonhypoxic (N) or hypoxic (H) conditions for 16 hr (lanes 1–6 at right) as follows: (1) (bold) increased expression in hypoxic Hif1a+/+ cells, loss of induction in Hif1a+/? cells, and loss of basal and induced expression in Hif1a?/? cells; (2) (bold and italicized) no effect of hypoxia on expression in Hif1a+/+ cells but decreased expression in hypoxic Hif1a+/? and Hif1a?/? cells; (3) (italicized) no effect of hypoxia on expression in Hif1a+/+ cells but decreased expression in hypoxic and nonhypoxic Hif1a?/? cells; (4) (plain) no effect of hypoxia or HIF-1α deficiency on expression. mRNA expression in Hep3B cells was also assayed (lanes 7,8). The indicated genes encode the following proteins: (GLUT1 and GLUT3) glucose transporter 1 and 3; (HK1 and HK2) hexokinase 1 and 2; (GPI) glucosephosphate isomerase; (PFKL) phosphofructokinase L; (ALDA and ALDC) aldolase A and C; (TPI) triosephosphate isomerase; (GAPDH) glyceraldehyde-3-phosphate dehydrogenase; (PGK1) phosphoglycerate kinase 1; (PGM) phosphoglucomutase; (ENO1) enolase 1; (PKM) pyruva