干血斑样本采集运输讲.ppt

可能的原因： 1. 血液未渗透滤纸或未填满圆圈前将滤纸移走； 用毛细管转移血液; 在取样前或后用戴手套或不戴手套的手触摸滤纸，滤纸上粘有洗手液； 毛细管或其他锐器移取血液 * Puncture Ring or middle finger palmer surface- approximately half way between the center of finger and side or tip, with a sterile lancet. * Puncture the patients finger with a sterile lancet or autolet. Dispose of the used autolet in the appropriately labeled sharp’s container. * Wipe away the first drop of blood with a clean gauze pad. The first drop of blood often contains excess tissue fluids which can alter the results of the test. Gently massage the patients finger to establish the flow of blood and to create a small bubble of blood to sample. Do NOT squeeze the patients finger as tissue fluid may dilute the sample. * For collection into a capillary tube, turn the patient’s hand over so that the blood drop forms toward the floor. Establish a bubble of blood for sampling. Holding the collection tube at an angle of about 10 degrees below the collection site, touch the tapered end of the tube into the bubble of blood. Capillary action will cause the blood to fill the tube. As the blood starts entering the tube be careful not to allow air bubbles to enter. Avoid collecting smeared blood and do not collect blood that has run into the cuticle or nail area. * For collection on filter paper, lightly touch the paper to a large blood drop. Allow the blood to the blood to soak through and completely fill the circle with a single application of blood. Apply blood to one side of the paper only. Fill remaining circles in the same manner. 婴儿艾滋病早期诊断滤纸片干血斑样本的采集、处理运输、保存及实验室检测 山西省妇幼保健院 张玉萍 2015-6-1 什么是干血斑（Dried Blood Spot DBS） 采集用EDTA抗凝的静脉血混匀滴在滤纸片上制备干血斑； 直接用患者的全血制备； 内 容 所需设备； 怎样采集和干燥DBS； 怎样包装和保存DBS； 有效和无效的DBS； 实际操作； 样本采集过程中的职责 采集有效的样本； 在被检测前正确地标记和保存样本； 保证样本记录正确长久保存； 避免抄写错误； 样本采集和检测结果一样重要！ 采集样本所需要的设备 血样本采集卡 片(滤纸片)； 可密封的塑料袋； 湿度指示卡； 干燥剂（1-2g/袋）； 怎样采集末梢血DBS 应用通用的安全防护措施； 清晰准确地在卡片上标记识别码； 完成针刺指尖程序； 均一地使每个印圈饱和地吸附血液； 两个充分吸附血液 的印圈比五个不完全吸附