跟有母性影响遗传基因的连锁定位系的构建.pdf

HEREDITAS (Beijing) 2007 11 , 29(11): 1393―1398 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1393 代方银, 谭端, 童晓玲, 胡海, 鲁成, 向仲怀 , 400716 : 母性影响遗传基因由于其杂交后代的表型受母本基因型的影响, 而不能直接反映当代个体的基因型, 这 给连锁定位测交亲本( 三隐性或双隐性系统) 的培育带来困难, 从而影响这类基因的定位研究。设计了一套杂交 培育方案, 其核心是使母性影响遗传基因先纯合, 再使非母性影响遗传基因纯合。采用所设计的方案, 成功培 育了家蚕第 13 连锁群的赤蚁(ch) 、无鳞毛翅(nlw, 新突变)和褐色卵t (b-t, 母性影响遗传) 的三隐性系统, 并培育 了第19 连锁群的狭胸(nb)和第二肾形卵(ki-2, 母性影响遗传, 待定位突变基因) 的双隐性系统。 : 母性影响遗传基因; 三隐性系统; 双隐性系统; 培育方案; 家蚕 Construction of linkage localization lines containing genes with ma- ternal effects DAI Fang-Yin, TAN Duan, TONG Xiao-Ling, HU Hai, LU Cheng, XIANG Zhong-Huai The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China Abstract: Since the phenotypes of hybrid progenies involving genes with maternal effects are affected by the geno- type of female parent, they cannot reflect the genotypes of individuals. This makes it difficult to develop test cross parents (triple or double recessive lines) for linkage localization and, consequently, hinders the progress in localiza- tion researches for these types of genes. In this study, we designed a set of hybridization schemes, the key of which was to make the “maternal-effect” genes homozygous at first, and then the non-maternal-effect genes. Using this scheme, we successfully produced a triple recessive line for genes ch (chocolate), nlw (non-lepis wing) and b-t (ma- ternal brown egg of Tsujitan) on the 13th linkage group and a double recessive line for genes nb (narrow breast) and ki-2 (kidney-shaped egg 2) on the 19th linkage group of Bombyx mori . Keywords: genes with maternal effects; triple recessive line; double