MEEKC、MEKC分别测定雷公藤有效成分与部分中药对映体的毛细管电泳新方法-研究.pdf

Abstract knownas Capillaryelectrophoresis(CE)，alsohi。曲performancecapillaryelectrophoresis all inrecent hasbeenused (HPCE)，isanalyticaltechnologydevelopingrapidly years．CE inthefieldof and sciencesenvironmental widely food，pharmaceutics,life monitoring thesisisfocusedon sciences，etc．This developingsimple，rapid，sensitive，reliablequalitative and methodsforthe of controlsomeChinesetraditionalmedicine quantitive quality by thesis consistsoftwo describedbelow： capillaryelectrophoresistechniques．The parts，as Partone ofMEEKCfor Application determinationofsome in componentTripterygium Hook．￡ wilfordii 1：Anovelmethod Chapter ofmieroemulsionelectrokinetic was establishedforthedeterminationof and the triptolidetfiptonide simultaneously．Instudy， theeffectof andconcentrationof andbufferwere type surfactant,cosurfactant，oil phrase pH investigated onthe microemulsionwas of systematicallyseparation．Theoptimized composed l％(w／v)sodium acetateand dodecyl sulfate，3％(v／v)n-butanol，】％(v／v)ethyl96％(v／v)5 mMborax-10mM at 8．5．Witha phosphate-sodiumpH voltageof25kV,detection