HPL法分析不同产地生何首乌中的蒽醌类化合物.doc

HPLC法分析不同产地生何首乌中的蒽醌类化合物 潘文嘉（通讯 ｝林双黎文敏林坚涛（广东医学院广东东莞523808） 】目的：采用反相高效液相色谱法测定十个已知产地的生首乌水解前、后 蔥醌类成分的含量，从而评价不同釆集地生何首乌的质量。方法：HPLC法，以 C18为色谱柱，甲醇-0.1%磷酸（85： 15）为流动相，流速为1.0mlbull;min-l; 检测波长254nm;结果：四川峨眉山样品中的蒽醌类成分含量最高，游离型和结 合型大黄素含量分别为235.59mg/100g, 18.17mg/100g,游离型大黄素甲醚为 97.69 mg/100g；而重庆缙云山、贵州施秉、广丙田阳样品中蒽醌类成分含量则 较低。结论：各产地生何首乌中蒽醌类成分主要为大黄素，大黄素甲醚，其含量 随产地差异较大，为生何首乌质量评价提供了可靠的指标和方法。 关键词】何首乌蔥醌HPLC法 】R284 】A 】2095-1752 （2012） 36-0131-03 Determination of anthraquinoid compounds in polygonum multiflorum produced in different areas by HPLC Wenjia,Pan*;Shuang/ Lin; Wenmin, Li; Jiantao, Lin Guangdong medical college,dongguan, 523808 corresponding author Abstract】 Objective: To determine the contents of anthraquinone before and after hydrolysis in the commercial medicinal materials of Radix Polygomi Multiflori collected from different areas respectively by RP-HPLC. Methods: The determination was carried out using C18 column, with mobile phase consisted of methanol-0.1% phosphoric acid solution（85:15）,the detection wavelength was 254 nm. Results: Anthraquinone contents in Sichuan Emei Mountain sample is maximal.The free type and union type emodin contents were 235.59mg/100g， 18.17mg/100g and the free type physcion was 97.69 mg/lOOg .The contents in the Chongqing Jin Yun county, Guizhou Shi Bing county, Guangxi Tian Yang sample were lower.Conclusion: Anthraquinone contained in the samples were mainly emodin and physcion.The contents varied greatly in the samples from the different producing areas. Make a Radix Polygomi Multiflori quality evaluation has provided the reliable index and method. Keywords】 polygonum multiflorum anthraquinone HPLC 何首乌始载于《开宝本草》，为寥科植物何首乌Poamp;gonum mul tiflorumThunb.的干燥块根。该植物为多年生缠绕性草本，块根多呈纺锤形，表 面红棕色至暗褐色，断面紫红色，故又称为赤首乌［1】。其性微温，味苦、甘、 涩。主要含有蒽醌类、二苯乙烯苻类和磷脂类等化学成分［2】。生首乌能解毒、 消痈、润肠通便，治瘰疡疮痈、风疹瘙痒、肠燥便秘［3］。临床主要用于调血脂、 强心、抗菌、抗癌、提高免疫功能等作用［4】。 何首乌的种植分布广泛,主产于河南、湖北、广西、广东、贵州、四川、 江西等地［5］。不同产地的气候、湿度、温度、土壤成分、栽培技术等条件各异， 再加上采收时间的不同，造成各产地的何首乌的化学成分在种类、含量上冇所差 异。并且由于野生资源紧缺，人工栽培品日益增多，