《花生茎水溶性膳食纤维（SDF）的提取工艺研究》》-毕业论文.doc

- PAGE 7 - 本科毕业论文 (设计) 题 目：花生茎水溶性膳食纤维（SDF）的提取工艺研究 学 院： 医 学 院 专 业： 食品科学与工程 姓 名： 指导教师： 年 6 月5日 花生茎水溶性膳食纤维（SDF）的提取工艺 研究 摘要 采用单因素和正交实验探讨了酶解法提取花生杆中水溶性膳食纤维（SDF）的提取工艺。木瓜蛋白酶预处理的花生杆粉的最佳提取工艺条件为：纤维素酶浓度为1.0㎎／ml，温度为40℃，pH值为4.8，水解时间为1小时。采用上述最佳提取工艺制备SDF，得到的样品的酶解率为13.4％，聚合度为1.0036，SDF的得率为1.25％。中性蛋白酶预处理的花生杆粉的最佳提取工艺条件为：纤维素酶浓度为0.5㎎／ml，水解温度为40℃，pH值为4.4，水解时间为0.5小时。采用上述的最佳工艺条件制备SDF，得到的样品的酶解率为14.10％，聚合度为1.1435，SDF的得率为2.23％。论文还研究了SDF的抗氧化活性。实验结果表明，两种蛋白酶预处理的花生杆粉制备的SDF均具有清除羟自由基、超氧阴离子自由基、DPPH自由基、铁还原能力和钼还原能力的抗氧化活性。 关键词 木瓜蛋白酶；中性蛋白酶；纤维素酶；水溶性膳食纤维；抗氧化活性 Study on the technology of extracting soluble dietary fibre from peanut powder Abstract Single factor and orthogonal design of enzyme extracted under peanuts in water-soluble dietary fiber (SDF) of the extraction process. Papain pretreatment of peanut powder under the optimum extraction conditions are as follows: the concentration of 1.0 Cellulase ㎎ / ml, temperature 40 ℃, pH value of 4.8, the hydrolysis time is 1 hour. The best extraction process using the above-mentioned Preparation SDF, the enzyme samples obtained was 13.4% for the degree of polymerization of 1.0036, SDFs a rate of 1.25 percent. Pretreatment neutrase peanut powder under the optimum extraction conditions are as follows: the concentration of 0.5 Cellulase ㎎ / ml, the hydrolysis temperature of 40 ℃, pH value of 4.4, the hydrolysis time of 0.5 hours. The best use of the above-mentioned preparation process conditions SDF, the enzyme samples obtained was 14.10%, polymerization degree to 1.1435, SDFs a rate of 2.23 percent. Paper also examined the antioxidant activity of the SDF. The experimental results show that protease pretreatment of the two peanut powder under the SDF are scavenging, superoxide anion radical, DPPH free radical, iron and molybdenum to restore the ability to restore the ability of antioxidant activi