《槲皮素与金属配合物的分光光度法研究》-毕业论文.doc

ANYANG INSTITUTE OF TECHNOLOGY 本 科 毕 业 论 文 槲皮素与金属配合物的分光光度法研究 Spectroscopic Study of Complexes of Quercetin with Metal 系（院）名称： 化学环境与工程系 专 业 班 级 ： 化学工程与工艺 学 生 姓 名 ： 学 生 学 号 ： 指导教师姓名： 指导教师职称： 讲师 槲皮素与金属配合物的分光光度法研究 摘要 利用紫外分光光度法研究槲皮素与Fe（Ⅲ），Co（Ⅱ）形成配合物的最佳反应条件，测定了其配比和条件稳定常数。在pH=4.05的HAc-NH4Ac缓冲溶液中，槲皮素与Fe（Ⅲ）配位，该配合物在426nm处有最大吸收。Fe3+的浓度在2.0×10-5—1.8×10-4 mol/L之间与吸光度成线性关系。采用等摩尔连续变化法测得槲皮素—Fe（Ⅲ）配合物的配位比为2:1，计算得到配合物的条件稳定常数为3.13×107。在pH=4.7的HAc-NH4Ac缓冲溶液中，槲皮素与Co（Ⅱ）配位，该配合物在584nm处有最大吸收。Co2+的浓度在2.0× 10-5— 1.1×10-4 mol/L之间与吸光度成线性关系。采用等摩尔连续变化法测得槲皮素—Co（Ⅱ）配合物的配位比为2:1，计算得到配合物的条件稳定常数为7.38×1011。 关键词：槲皮素 Fe（Ⅲ）配合物 Co（Ⅱ）配合物 分光光度法 Spectroscopic Study of Complexes of Quercetin with Metal Abstract The best formative conditions of complexs of quercetin and Fe（Ⅲ）,Co（Ⅱ）were studied by Spectrophotometry,the molar ratio and the conditional stability constant were detected .The results showed that in the buffer solution of HAc-NH4Ac( pH =4.05),quercetin reacted with Fe（Ⅲ）to form a complex (λmax=426nm), A linear relationship holds between the absorbance and the concentration of Fe（Ⅲ）in the range of 2.0 × 10-5—1.8 × 10-4 mol / L. Using equimolar continuous variation method measured the ratio of quercetin:Fe (Ⅲ) in the complex was 2:1, the calculated conditional stability constant of the complex was 3.13 × 107. In the buffer solution of HAc-NH4Ac( pH = 4.7), quercetin reacted with Co（Ⅱ）to form a complex (λmax=584nm) , A linear relationship holds between the absorbance and the concentration of Co（Ⅱ）in the range of 2.0 × 10-5—1.1 × 10-4 mol/L.Using equimolar continuous variation method measured the ratio of quercetin: Co（Ⅱ）in the complex was 2:1, the calculated conditional stability constants of the complex was 7.38× 1011. Key words Quercetin ； Quercetin – Fe (Ⅲ) Complex ； Quercetin –Co (Ⅱ) Complex ； Spectrophotometry PAGE 目录