丹红注射液预处理对大鼠肝脏缺血再灌注损伤保护作用-外科学(肝胆外科)专业论文.docx

福建医科大学 福建医科大学 2008 级硕士研究生毕业论文 - - PAGE 4 - At 4、12、24 hour after reperfusion，Serum ALT、AST levels were significantly higher in the IR group compared with those of the Sham group（P＜0.05），and pathologic injury was more severe than the Sham group；In the DH group，Serum ALT、AST levels were markedly decreased by pretreatment with Danhong injection compared with those of the IR group（P＜0.05），and the abnormal morphological changes of hepatocytes were diminished remarkably. 2． Influence of Danhong injection pretreatment on serum cytokines levels after hep- atic ischemia-reperfusion. At 4、12、24hour after reperfusion，Serum TNF-α and IL-6 levels were significantly higher in the IR group compared with those of the Sham group（P＜ 0.05）. Serum TNF-α and IL-6 levels were markedly reduced by pretreatment with Danhong injection compared with those of the IR group（P＜0.05）. 3. Influence of Danhong injection pretreatment on expression of ICAM-1 after hepatic ischemia-reperfusion. Immunohistochemical staining showed that：In the Sham group， ICAM-1 expression of liver tissue was negative；In the IR group，at 4、12、24 hour after reperfusion ICAM-1 expressions in liver tissue were significantly increased in sinusoidal endothelial cells and small arteries muscle，small vein endothelial cells of portal area，part of the liver parenchymal cells could also be found the expressions of ICAM-1（P＜0.01）；In the DH group，At three time points after reperfusions ICAM-1 expressions in sinusoidal endothelial cells and liver cells were significantly reduced compared with the Sham group（P＜0.01）. Conclusion 1．Danhong Injection pretreatment could significantly reduce the levels of serum ALT、AST，ameliorated the hepatocellular damage and protected liver function. 2．Danhong injection pretreatment significantly decreased the levels of serum TNF-α、IL-6，reduced liver injury following hepatic ischemia reperfusion. 3．Pretreatment with Danhong injection could markedly inhibit the liver over- expression of I