番茄红素的超声波辅助提取以及纯化研究食品科学专业论文.docx

II II Study on the Ultrasound-assisted Extraction and Purification of Lycopene Abstract In this paper technology of extraction and purity of lycopene was studied with the improving methods of ultrasound-assisted extraction and solvent extraction, and using tomato ketchup as material, in which the lycopene of resin with the effect of saponification was purifying by Column–chromatography and recrystallization. For the purpose of enriching the theoretical basis and providing better methods of purifying content of lycopene, and saving the extraction time and increasing the extraction rate, cutting down the waste of material. The conditions of extraction and purity was determined in saponification experiment by using the response surface methodology .The following points are main conclusions of those studies: The optimum conditions for ultrasound-assisted extraction were:ultrasonic output power of 620.54W, extraction time:23.76min, extraction temperature:47.60℃，the maximum extraction yield prediction was 66.49mg/100g in these conditions, Considering the actual condition, it can be adjusted to ultrasonic output power of 620W, extract time 24min, extract temperature 48℃，The extraction yield was 66.49mg/100g, the extraction rate can reach a rate of 89.19% one time. The optimum combination was A1B2C2 in saponification, that is,extract temperature:50℃, extract time:90min, additive amount of lycopene of resin:1.0g. X-5macroporous adsorption resin was determined as adsorbent column chromatography in the purification of lycopene in experiment; With the conclusion that the Freundich adsorption equation is better than Langmuir equation, the adsorption of X-5 resin adsorption on lycopene is not simply molecular layer adsorption, the Lycopene adsorption process by resin can be analyzed according to the Freundlich adsorption equation; According to the static adsorption experiment, adsorption and desorption at normal temperature with the optimal concentration of 0.0008g/mL