代谢工程方法改造大肠杆菌生产胸苷-发酵工程专业论文.docx

万方数据 万方数据 thymidine. While BS08 containing two plasmids produced 272 mg thymidine/L. In fed-batch fermentation, BS08 accumulated 1248.8 mg thymidine/L with a yield of 12.5 mg/(g·glucose) and a productivity of 15.61 mg/(L·h). The experimental results showed that metabolically engineered strain E.coli has potential applications for thymidine production. KEY WORDS ： Escherichia coli, thymidine, de novo pathway, salvage pathway, fed-batch fermentation 目 录 第一章 文献综述.......................................................................................................... 1 1.1 胸苷的性质及应用.......................................................................................... 1 1.2 胸苷的生产工艺............................................................................................. 1 1.3 大肠杆菌中胸苷合成途径及其代谢调节机制............................................. 2 pyrBI 和 pyrE 的弱化子调控 4 pyrC 和 pyrD 的调控 5 carAB 的调控机制与 reiterative transcription 6 1.4 代谢工程......................................................................................................... 7 1.5 胸苷的研究进展............................................................................................. 9 1.6 本课题研究意义和主要研究内容................................................................ 10 第二章 实验材料和方法............................................................................................ 12 2.1 实验材料........................................................................................................ 12 2.1.1 菌种和质粒......................................................................................... 12 2.1.2 主要实验仪器..................................................................................... 13 2.1.3 实验药品............................................................................................. 13 2.1.4 主要溶液配置..................................................................................... 14 2.1.5 培养基................................................................................................. 15 2