成年和老年大鼠脑出血后海马齿状回神经干细胞增殖及分化的研究人体解剖与组织胚胎学专业论文.docx

宁夏医科大学硕士研究生学位论文 中文摘要 成年大鼠 BrdU/NeuN 阳性细胞数较老年多，老年大鼠 BrdU/GFAP 阳性细胞数较成年多。 结论 ①正常大鼠的海马齿状回均有少量神经干细胞增殖，成年大鼠较老年大鼠 增殖能力强；②脑出血后大鼠的海马齿状回神经干细胞增殖增强，出血后 7 d 达到峰值， 脑出血后老年大鼠神经干细胞增殖能力明显低于成年大鼠；③脑出血后大鼠的海马齿 状回神经干细胞向神经元分化增多，其中成年大鼠 BrdU/NeuN 阳性细胞数较老年多， 老年大鼠 BrdU/GFAP 阳性细胞数较成年多；④年龄是影响脑出血后神经干细胞增殖分 化的重要因素。脑出血后，随着年龄的增长，脑内神经干细胞增殖和分化能力逐渐减弱。 关键词 脑出血，海马齿状回，神经干细胞，增殖，分化 The Study of Neural Stem Cells’ Proliferation and Differentiation on Dentate Gyrus after Intracerebral Hemorrhage in Adult and Aged Rats ABSTRACT Objective Observed proliferation and differentiation of dentate gyrus neural stem cells after intracerebral hemorrhage（ICH）in adult and aged rats. Discussion the variation of neural stem cells after ICH. Method 192 healthy Sprague-Dawley rats, 96 in adult rats and aged rats , either male or female. Each adult and aged rats were randomly divided into normal group（18 rats）, sham operation group（12 rats）and ICH model group（3 d, 7 d, 14 d, 21 d, 28 d, 66rats）. ICH model was established by injecting collagenase Ⅶ into the brain of rats. The normal group and sham operation group were killed in 7d, ICH model groups were killed in 3 d, 7 d, 14 d, 21 d and 28 d. All rats were sacrificed before 24h intraperitoneal injection with Brdu to mark proliferating cells. Behavior was scored by Bederson score Standard. Detected number of proliferating cells labeled by immunohistochemistry. Observed differentiation of proliferating cells marked by double target Brdu/NeuN and Brdu/GFAP. Results Score of neurological behavior in rats of sham operation group and the normal group had no difference in postoperative, hemorrhage group were symptom hemorrhage lesions contralateral hemiplegia, hemorrhage group were symptom hemorrhage lesions contralateral hemiplegia over time, Neurological function deficit of hemorrhage group gradually recovered. Water content of cerebral hemorrhage reached a peak at 72h and then gradually decreased, edema significantly reduce after 7d, resultd has Significant difference (P0.05)， IC