Fstl1在肺纤维化中通过CTGF调控肺成纤维细胞的-中国科技论文在线.PDFVIP

中国科技论文在线 Fstl1 在肺纤维化中通过CTGF 调控肺成纤 维细胞的增殖、迁移和分化 吴艳艳，董莺莺** 5 （苏州大学剑桥-苏大基因组资源中心，苏州，215123 ） 摘要：目的：研究在肺纤维化疾病中，Fstl1 通过CTGF 在肺成纤维细胞增殖、迁移和分化 中的作用。方法：免疫组化及qRT-PCR 检测博来霉素处理的野生型及Fstl1+/-小鼠肺组织、 肺成纤维细胞中CTGF 的表达；通过MTT 实验、划痕实验和Western blot 等，检测不同处 10 理组的野生型、Fstl1+/-小鼠肺成纤维细胞的增殖能力、迁移能力以及分化相关蛋白的表达差 异。结果：博来霉素处理的 Fstl1+/-小鼠肺成纤维细胞增殖能力明显低于正常对照组，伴随 着低表达的CTGF 蛋白；外源加入CTGF 蛋白能明显促进肺成纤维细胞的增殖、迁移及分 化，单倍缺失Fstl1 抑制CTGF 的作用，而外源加入Fstl1 蛋白明显促进了CTGF 在成纤维 细胞中的作用。结论：在博来霉素诱导的小鼠肺纤维化疾病过程中，Fstl1 通过CTGF 促进 15 肺成纤维细胞的增殖、迁移和分化，从而促进肺纤维化。 关键词：细胞生物学；肺纤维化；Fstl1；CTGF 中图分类号：Q28 Fstl1 regulate the proliferation, migration and 20 differentiation of lung fibroblasts through CTGF in lung fibrosis Wu Yanyan, Dong Yingying (Cambridge-Suda (CAM-SU) Genomic Resource Center, Soochow University, Suzhou 215123) Abstract : Objective: Studying the role of Fstl1 in the proliferation, migration and differentiation of lung 25 fibroblasts through CTGF during lung fibrosis. Methods: IHC and qRT-PCR were used to examine the protein and mRNA expression level of CTGF in lung tissue and lung fibroblasts from WT and Fstl1+/ - mice treated with bleomycin. MTT, wound healing and western blot methods were used to detect the proliferation, migration and differentiation of lung fibroblasts from WT and Fstl1+/- mice treated with exogenous CTGF and/or Fstl1 protein. Results: The Proliferation of lung fibroblasts and CTGF 30 expression level was significantly reduced in Fstl1+/- mice compared with WT control mice after bleomycin treatmentin.