氧化亚铁硫杆菌中磁小体形成相关基因mpsA在不同铁.pdf

40 6 () Vol.40 No.6 2009 12 Journal of Central South University (Science and Technology) Dec. 2009 mpsA ( 410083) (Acidithiobacillus ferrooxidans) ATCC23270 ATCC23270 mpsA ORF1622 PCR mpsA 20 mmol/L FeCl FeSO ·7H O 3 4 2 ORF1622 PRK05724 mpsA 48%acetyl-CoA carboxylase carboxyltransferase subunit alpha mpsA mpsA PCR Q786 A 1672−7207(2009)06−1471−05 Real-time PCR analysis of different Fe ion shock responses of mpsA gene in Acidithiobacillus ferrooxidans ATCC 23270 LIU Xin-xing, LIU Wen-bin, YAN Ying, WU Hai-yan, QIU Guan-zhou (School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China) Abstract: The whole genome of the type strain Acidithiobacillus ferrooxidans ATCC 23270 and the conserved domain and amino acid sequences as well as the protein identity of the target gene were analyzed. To obtain further knowledge of the magnetosomes formation mechanisms of Acidithiobacillus ferrooxidans in response to different kinds of Fe ions, temporal gene expression profiles were examined in cells subjected to 20 mmol/L FeCl and FeSO ·7H O shock by using 3 4 2 reserved transcript PCR. The results indicate that ORF 1622 of the ATCC 23270 is homologous with mpsA gene in magnetotactic bacteria and the expressions of ORF 1622 is related to Fe2+. According to the fact that the Acidithiobacillus ferrooxidans is able to synthesize i