环江香猪抗腹泻性状和产仔数性状相关基因多态性研究-养殖专业论文.docxVIP

点的CT和TT基因型频率分别为O．136和0．864，有利等位基因C频 率为0．068。C位点检测出的AA、AG和GG三种基因型频率分别为 0．694、0．279和0．027，有利等位基因G频率为O．166。D位点检测出 GC和CC基因型频率分别为0．344和0．656，有利等位基因G频率为 O．172。环江香猪有利等位基因G与C频率均较低，这与环江香猪仔 猪断奶前腹泻死亡率高的调研结果相吻合。进一步对仔猪健康群体、 仔猪腹泻群体和仔猪腹泻死亡群体ABCD四个位点基因型研究显示， 四个位点的不同基因型在三个分组中分布均存在显著差别(PO．05)， 其中C位点差别极显著(PO．01)，说明检测出的四个位点的不同基因 型与仔猪腹泻抗性／易感相关。 2．环江香猪繁殖性状相关基因FSH B、ESR和ZARl基因的多 态性研究。结果显示，首次成功克隆测序了环江香猪ZARl基因的外 显子3，与GenBank中的ZARl基因(DQ23 1443，gi外 显子3序列100％同源。针对134头环江香猪检测样本中均未发现FSH B和ESR基因的多态突变位点。对ZARl基因的外显子3和部分内 含子3测序后发现5个新突变SNP位点，未见有己报道的对猪产仔 数产生显著影响的外显子3(C54T)突变。结果显示不同品种猪基因 突变多态性亦呈现为不同的模式，研究结果对建立环江香猪基因标记 辅助选择方法提供理论基础。 关键词：环江香猪仔猪腹泻产仔数候选基因单核苷酸多态性 II 万方数据  PoLYMoRPHISM ANALYSIS OF GENES ON THE TRAITlS OF PIGLET DIARRHEA AND LITTER SIZE OF SOWS IN HUANJIANG MINIArTURE PIG ABSTRACT Huanj iang miniature pig is a famous local variety in Guangxi，which is Chinese rare breeds，and through the national geographical indications of origin certification in 2003．Farmer and consumers are interested in excellent properties such as resistance to crude feed，cold，prolific，fresh meat and motherhood．However,Huanj iang pig and other small pig breeds are ignored since the reform and opening up，China has attached importance to solving the problem of food and clothing，aquaculture tends to feed large and grow fast pig species．In addition to the Huanjiang pig diarrhea piglet mortality,resulting in less amount of Huanj iang pig breeding and high cost．At present,the Huanjiang pig related functional gene research is rarely reported，the study bassed on candidate genes related to the ability of anti diarrhea and the litter traits of sows，including MUC 1 3，FSHl3，ESR,and ZARl．The polymorphism detection of these candidate genes by PCR-RFLP,HRM and direct sequencing assays．The details of results were described as follows： The polymorphism detection of MUC 1 3 related to piglet diarrhea III 万方数据  resistance／susceptibility trait in Huanjiang pig．The results showed that four of the five analyed SNPs A，B，C，and D loci