《钒酸盐氧化法和化学氧化法测定血清胆红素的比较》-毕业论文设计（学术).docVIP

PAGE 钒酸盐氧化法和化学氧化法测定血清胆红素的比较 摘要：目的 比较钒酸盐氧化法和化学氧化法测定血清胆红素的性能。方法 用钒酸盐氧化法、化学氧化法分别测定血清中总胆红素（TBil）和直接胆红素（DBil）。根据美国临床实验室标准化委员会（NCCLS）指南，比较了两种方法的精密度、线性范围、回收率和抗干扰能力，并计算两种方法测定结果的相关性。结果 两种方法重复性实验批内变异和批间变异分别小于3.0%和5.0%；两种方法测定TBil的平均回收率分别为100.17%、101.12%；测定DBil的平均回收率分别为99.36%、98.35%。钒酸盐法测定TBil的线性范围为0-570μmol/L，化学氧化法测定TBil的线性范围为0-650 μmol/L，钒酸盐法测定DBil的线性范围为0-312 μmol/L，化学氧化法测定DBil的线性范围为0-213 μmol/L。以钒酸盐方法为对比法（Y），对化学氧化法（X）进行评估，两种方法测定TBil回归方程为Y=0.995X+1.954，相关系数r2=0.999，（P＞0.05），测定DBil的回归方程为Y=0.940X+0.731，r2=0.996，（P＞0.05）。化学法抗溶血干扰能力较钒酸盐法强，而抗维生素C的干扰较钒酸盐法差，两种方法对TG的抗干扰能力相当。结论 钒酸盐氧化法和化学氧化法测定血清胆红素性能相当，两种方法的线性检测范围、精密度、回收率和抗干扰能力均符合临床常规使用要求。 关键词 总胆红素; 直接胆红素; 方法学比较 Comparison of vanadate-acid oxidizes method and chemical oxidizes method to analyze bilirubin in serum Abstract Objective To compared the performances of the vanadate-acid oxidizes and chemical oxidizes methods detect bilirubin in serum. Method The total bilirubin and direct bilirubin in serum were analyzed by the methods of the vanadate-acid oxidizes and chemical oxidizes, respectively. The precision, linearity, rate of recovery, anti-interference ability of two methods were compared according standard method of NCCLS, and calculate the relativity of two methods. Results The within-run and run to run imprecision (CV) of two kinds of methods were less than 3.0% ands 5.0%, respectively. The recovery rate of two kinds of methods detected total bilirubin were 100.17%、101.12%，respectively， and detected direct bilirubin were 99.36%、98.35%，respectively. The linear range of TBil measured by the method of the vanadate-acid oxidizes was 0-570 μmol/L and that the chemical oxidizes was 0-650μmol/L, the linear range of DBil measured by the method of the vanadate-acid oxidizes was 0-321μmol/L and that the method of the chemical oxidizes was 0-213μmol/L, respectively. The TBil levels by vanadate-acid oxidizes method (X) were closely correlated with those obtained chemical oxidizes method (Y), Y=0.995X+1.954, r2=0.999