《酵母细胞壁多糖提取工艺研究》-毕业论文设计（学术).doc

PAGE 精品 摘 要 本实验采用的 是CICC编号31229的 红酵母，通过对红酵母的实验，了解红酵母的特性及生长特点，进而改善红酵母的培养条件及培养基来扩大红酵母的生长浓度。研究从红酵母中提取酵母多糖的提取工艺，确定酵母多糖提取方法。 采用摇瓶培养的方法，通过碳源、氮源及主要营养与环境条件等对红酵母产菌量的影响试验，以及葡萄糖、蛋白胨、酵母膏三因素三水平的正交试验，获得了红酵母的最佳生长条件：葡萄糖2％，蛋白胨1．0％，酵母膏0,5％，磷酸二氢钾0．15％，硫酸镁0．01％，氯化钠1．0％，初始pH5．0，接种量10％，500mL三角瓶中培养基装量为80mL，发酵温度为28℃，摇床200r／min。在此优化的培养条件下，海洋红酵母的产菌量可达9．7亿个／mL，比优化前发酵培养基产菌量（5．6亿个／mL）提高了73．2％，比单因素试验结果（8．1亿个／mL）提高了19．8％。 研究从酵母中提取胞壁多糖的提取工艺。提取工艺路线为：酵母溶解—冻融一超声波破碎一碱溶一中和一沉淀一洗涤—烘干。通过正交试验对酵母破壁和碱溶条件进行优化。初步寻求提取工艺条件，多糖得率为19．4％，用苯酚一硫酸法测定多糖的含量为51．9％。 关键词: HYPERLINK javascript:GJCSearch(海洋红酵母); 红酵母 HYPERLINK javascript:GJCSearch(摇瓶); 高细胞密度 培养条件优化 酵母多糖 提取工艺 Abstract The experiment was through the study of the Rhodotorula glutinis，to understand the characteristics and growth characteristics of Rhodotorula glutinis, Thereby improving conditions for the cultivation of red yeast and the medium to expand the growth of red yeast concentration. A flask of training methods，The use of Rhodotorula glutinis，Through the carbon source, the nitrogen source and the main nutrition and the environmental condition and so on produce the fungus quantity to the Rhodotorula glutinis benthica the influence experiment, as well as the sucrose, the protein peptone, the yeast paste three factors three levels orthogonal experiments, we have obtained the Rhodotorula＇s best fermentation condition：sucrose 1.5 %, protein peptone 1.0 %, yeast paste 0.5 %, potassium dihydrogen phosphate 0.15 %, magnesium sulfate 0.01%, sodium chloride 1.0 %, initial pH 5.0, inoculating quantity 8 %, 80 mL medium in the 500 mL triangle bottle, temperature 28℃, 200 revolutions/minute. Under this optimized condition, the number of Rhodotorula has reached 970 million / mL, which enhanced 73.2 % to the initial conditi（560million/mL） and improved 19.8 % to the result of the test of the single factor （810 million/mL）. This experiment is studying on the craft of polysaccharides extraction from rhodotorul cell-wal1．The craft route drawn：yeast dissolv