红平菇液体菌种栽培技术和营养成分分析与多糖提取工艺-森林保护学专业论文.docxVIP

东北林业大学硕士学位论文 H20：脱色得到稍呈淡黄色的红平菇多糖。多糖功能的试验结果表明，红平菇多糖对羟 基自由基和超氧离子自由基都有清除作用，对超氧离子自由基的清除作用较好；但是对 大肠杆菌(＆而e一如扭∞ff)DH5a的抑制作用不明显，对黑曲霉(爿印e俘池s嘲弘，)无 抑制作用。 关键词红平菇；二级液体菌种；栽培技术；营养成分；红平菇多糖  Abstl?act ne cultivating tecllnique of liquid isolate in Harbin area，nutrition coInPosition analysis and p01ysaccharide abstracting technics of fruiIing body off垤Ⅱ，Dms《加Ⅲ甜、Vere studied iIl t11is papeL The cultivating technique included the cultuIe characteristics of hyphae，screening of first-class isolate culture medium，and screening of 1iqu讯isolate culture medium，pmduction of cultiVating isolate and maIlagemenf technique of mushroom gro州h．Many ifems of nutrition composition of．础e“，口f“s硅f口珑Dr fruiting body cultivated were analysed，polysaccharide abstIacting technics and polysaccharide function of it was studied．Tbtal rcsults are as foIlOws： (1)Results of the culture characteristics and the screening of first-class isolate culture medium indicated that both of me two tested culture media were fjt forthe growth of州P“rD胁5 加mDr mycelium，hyphae伊ew Very well，wllite，Villifonn，cotton wool shape to powder shape． The mycjlium of PkHron岱加卅Dr could produce extracellular phenol oxidase．From the point of economical view，PDA was the most suitable first-dass isolate cuhure medium for州e“ro础5 《尬用or．The optimum temperature for the growth ofhyphae was 25℃～28℃，the opfimum pH is 6～7． 5 kiIlds of IiquidⅡledia were used in the cultIlring of second-dass isolate．Throu曲me contrasts on fhe hyphae mass of liquid isolate，hyphae’s伊owth condition aIld days of oVergrowing the nutrient nuid，concluded that me optimum liquid media for f沁“rDm5面口ⅢDr werc cornmeal—dextrose-peptone and bran—dextrose-peptone media，mycelia could over掣ow the nutdent fIuid in 7 days． (bntrastiIlg second-dass liqujd isolate as mother isolate for弛“加ms咖，，zDr to produce cultivating isolate wich s01jd orjginal isolate as mother isolate，the fo肿er had the merit of hyphae restoring quidkl y，hyphae growing fast in solid medjum，hyphae咎owing well and hyphae伊owing