LL37和IL8基因及蛋白在息肉组织中的表达和意义.docx

中文摘要 织中的阳性表达率明显高于在下鼻甲组织中的阳性表达率(P0．01)。 (3)LL一37 mRNA与IL一8mRNA在鼻息肉组织的表达呈正相关性 (r_0．524，P0．01)，LL-37蛋白与IL-8蛋白在鼻息中的表达亦呈正 。相关(pearsonX2=19．954，P0．01)。(4)LL-37 mRNA和蛋白在临床II 型ⅡI期及Ⅲ型组的表达水平与II型I期及II型II期组无明显差异 (PO．05)，IL-8mRNA和蛋白在临床II型ⅡI期及ⅡI型组的表达水平明 显高于II型I期及II型II期组(PO．01)。 结论：(1)LL-37和IL-8在正常鼻粘膜中起一定的免疫作用。(2) LL-37和IL．8在鼻息肉组织中的表达明显高于正常下鼻甲组织，二者 均可能参与鼻息肉的形成和发展。(3)IL-8在鼻息肉组织中的表达可 能反应鼻窦炎的严重程度。(4)在鼻息肉组织中，LL-37与IL-8的表 达呈正相关提示LL-37可能通过扩大后天免疫反应来提高机体的免 疫功能。 关键词：LL-37，IL-8，mRNA，蛋白质，鼻窦炎，免疫组化 ll  硕士学位论文 英文摘要 AB STRACT obj ective：Nasal polyp is a common chronic inflammatory disease of nose and sinus．The pathophysiological mechanisms of nasal polyp remain poorly understand．This study is aimed to investigate the expressions of LL-37 and IL一8 in nasal polyp tissues and their significances． Methods：Thirty—one nasal polyp tissues from patients with chronic sinusitis who accepted functional endoscopic sinus surgery(FESS)were randomly selected in our hospital．Another 11 inferior turbinate specimens from patients with the deviation of nasal septum who were performed corrective procedure served as controls．The expression level of LL-37 and IL-8 mRNA were analyzed by semi—quantitative reverse transcription polymerase chain reaction(RT-PCR)，and the expressions of LL-37 and IL-8 protein were detected by immunohistochemical staining in nasal polyp and inferior turbinate tissues，respectively．Student’S t-tests， 圣．tests and Pearson correlation analysis were applied as statistics analytical methods． Results：(1)LL-37 mRNA was all positively expressed in 31 nasal polyps tissues and 1 1 inferior turbinate tissues by RT-PCR analysis． LL-37 mRNA was obviously over—expressed in nasal polyp tissues than that in inferior turbinate tissues(PO．01)．LL一37 protein was positively III  硕士学位论文 英文摘要 expressed in nasal polyp tissues of 29 cases(29／3 1，93．55％)，and in inferior turbinate tissues(5／11，45．45％)by immunohistochemical staining．There was significant difference between the positive expression rate of LL-37