Genistein磁性纳米微对胃癌细胞株SGC7901细胞增殖及凋亡的影响.docx

兰州大学硕士学位论文3、流式细胞仪检测不同浓度GEN．M-NPS作用72h后的细胞凋亡率分别是 兰州大学硕士学位论文 3、流式细胞仪检测不同浓度GEN．M-NPS作用72h后的细胞凋亡率分别是 5．62％(10pmol／L)、13．46％(201xrnol／L)、26．40％(40pmol／L)、37．34％(80pmol／L)、 49．43％(1001maol／L)，阴性对照组的凋亡率为2．60％；DNA凝胶电泳结果显示 40岬oFL组作用24h可见明显的凋亡细胞形成的梯状条带。 结论： Genistein制成磁性纳米微粒后，成球性好，但是载药量和包封率都不太高； 体外细胞试验中，GEN—M-NPS能有效地抑制胃癌细胞增殖并诱导细胞凋亡，与 Genistein相比，有缓释性。 关键词： 染料木黄铜／药理学；胃癌细胞；凋亡；．纳米微粒 兰州大学硕士学位论文Effect 兰州大学硕士学位论文 Effect of Genistein--Magnetic-·Nanoparticles on the Proliferation and Apoptosis of Gastric Cancer Cells Line SGC．790 1 ABSTRACT oBJEC。I．1VE： To synthesize Genistein-magnetic—nanoparticles(GEN-M-NPS)and investigate its microstructure and properties，to explore the effect of the GEN-M-NPS on the proliferation and cell apoptosis of gastric cancer line SGC一790 1． M哐THoDS： 1．GEN-M-NPS were prepared by the emulsion／polymer cross-linking technique． the．morphology and size of nanoparticles were observed by transmission electron microscope(TEM)；m loading rate and embedding ratio were confirmed by ultraviolet-spectrophotometer． 2．SGC一790 1 cells in logarithmic growth phase were treated with various concentrations of GEN-M-NPS and Genistein， MTT assay Was used to examine the inhibition mte through the absorbency of 24h，48h， and 72 h． 3．After 7901 cells were disposed by(讵N-M-NPS at different concentrations for 72h，the cellular apoptosis rate was quantified by flow cytometry．DNA fragmentation Was evaluated by agarose gel electrophoresis at the level of drug concentration(40rtrnol厂L)． RESULTS： 1．GEN-M-NPS were round or elliptical，and the diameter Was about 1 05 nnl， drug loading rate Was aboutl7．28％and embedding ratio Was’10．33％． 2．GEN-M-NPS could apparently inhibit the proliferation of 790 1cell in a time and dose dependent manner妒O．05)．On the increase of time the inhibitive effect III 兰州大学硕士学位论文raised 兰州大学硕士学位论文 raised from 1 1．35％to 53．53％in 48h．On the increase of drug concentration the inhibitive effect raised from 38．61％to 86．07％．GEN．M二NPS have characteristic of slower release in