COX2P53基多态性及幽门螺旋杆菌感染与胃癌高发区胃癌关系的研究.docx

优秀毕业论文 精品参考文献资料 兰州大学硕士研究生毕业论文Pro 兰州大学硕士研究生毕业论文 Pro等位基因(Arg／Pro+Pro／Pro基因型)者在正常对照组和胃癌前病变组以及在胃 癌组和胃癌前病变组之间均无统计学差异(P0．05)，但在正常对照组和胃癌组中有 统计学差异(0R：1．846,95％CI：1．006-3．387,P=0．046)。分层分析显示携带P53eodon Pro 等位基因主要增加吸烟和Hp感染者患胃癌的风险，OR值分别为2．663(1．193～5．946) 和2．415(1．027--√5．676)。 结论1．COX．2．899GC的C等位基因与胃癌高发区甘肃河西地区胃癌发病的风险增 加相关并与H．pylori感染有协同作用。 2．COX．2．899GC的C等位基因可能增加胃癌高发区甘肃河西地区胃癌前病变发展 为胃癌的风险。 3．COX．2codon587GA与胃癌高发区甘肃河西地区胃癌发病的风险无关。 4．P53的Pro等位基因与胃癌高发区甘肃河西地区胃癌发病的风险有关，主要增加吸 烟和H．pylori感染者患胃癌的风险。 关键词环氧化酶．2；P53；基因多态性；幽门螺杆菌；胃癌；癌前病变 n 兰州大学硕士研究生毕业论文Study 兰州大学硕士研究生毕业论文 Study on the relationship among genetic polymorphisms of COX．2 and P53， H．pylori infection and susceptibility to gastric cancer in high incidence area Objective 1．To study the distribution of COX-2—899GC，587GA and 53CD72 GC gene polymorphisms among patients with gastric cancer and patients with precancerous lesion and health control in hight evidenve area,Hexi，of Gansu Province in China．2．To investigate the relationship among COX一2-899GC，587GA and P53 CD72 GC gene polymorphisms，H．pylori infection and susceptibility to gastric cancer in hight evidenve area，Hexi，of Gansu Province in China． Methods Subects(in Hexi area)were divided into three groups．140 gastric cancer patiens，1 10 precancerous lesion patients and 125 control subjects were enrolled．Patients with gastric cancer were recruited by pathological diagnosis．Patients with precancerous lesion were screened by gastroscope biopsy and pathological diagnosis．The control subjects were excluded gastric disease by gastroscope．COX一2-899GC，COX一2 codon 587GA and P53 codon Ar972Pro polymorphism Was analyzed by PCR—TaqMan probes． H．pylori in gastric mucosa was detected by Warhin-Starry silver staining．The results were analyzed by SPSSl3．0． Result The frequency of COX·-2·-899C carrier genotype in gastric cancer patients Was not only significantly higher than that in control health subjects(尸=O．029，OR=1．956，95％ CI：1．067-3．586)，but also significantly higher than that in gastric precan