Ghrelin腹内侧核调节大鼠胃运动及迷走复合体诱发弓核c-Fos蛋白表达-生理学专业毕业论文.docx

Effects Effects of ghrelin in VMH on gastric motility and in DVC on immunoreactive expression of c-Fos Protein in ARC in rats Abstraet objective： To investigate the effects of ghrelin in VMH gastric motility and its mechanism by which hypothalamic ghrelin is involved in the regulation of gastric motility,and investigate the effects of DVC administration of ghrelin the immunoreactive expression of c—Fos protein in ARC in rats． Methods：recording of gastric motility in conscious rats and immunohistochemistry Result： (1)．The amplitude of gastric motility in conscious rats were dose-dependently increased by microinjection of ghrelin into VMH，and the effects were abolished by vagotomy． (2)．The amplitude of gastric motility Was not increased by microinjection of the GHS—R antagonist(D．Lys3-GHRP．们and ghrelin into VMH． (3)DVC injection of ghrelin l Opmol ghrelin 20pmol dose-dependently induce the expression of c—Fos protein in ARC． Conclusion： Our results suggest that extro-VMH ghrelin may be involved via GHS—R in the regulation of gastric motility,implying that ghrelin has functional resemblance to motilin in the central regulation of gastric motility．DVC injection of ghrelin induces the expression of c-Fos protein in ARC，and tlley dose-dependent，which indicates that except ARC，one of the other possible regions where ghrelin exerts its orexigenic effect is DVC． Postgraduate：Yan Qu(Physiology) Supervisor：Prof．Zheng-Yao Jiang Key Words：Ghrelin；；gastric motility；ventromedial hypothalamus nucleus； arcuate nuclei；dorsal vagal complex；c—fos protein 青岛人学硕一I：学位论文英文缩略词表 青岛人学硕一I：学位论文 英文缩略词表 AgRP Agouti related protein 刺鼠相关蛋白 ARC Arcuate nucleus 弓状核 a．MSH a-melanocyte．stimulating hormone 黑色素细胞刺激素 CART Cocaine and amphetamine regulated可卡因一安非他明调节 transcript 转录肽 DVC Dotal vagal complex 迷走复合体 GHS．R Growth hormone secretagogue receptor 生长激素促分泌物受体 LHA Lateral hypothalamic area 下丘脑外侧区 MT Motilin 胃动素 NPY Neuropeptide Y 神经肽Y NS Normal saline 生理盐水 POMC Pro-opiomelanocortin 阿黑皮素原 PVN Paraventri