Mir30b抑制Oxr在鸡缺硒性心肌细胞凋亡中作用机制的研究.docx

CONTENTS CONTENTS Abstract iII Chinese ．I Abstract in English ．． ． ．．111 1 Introduction 1 1．1 The research progress of selenium deficiency on the heart damage ．1 1．1．1 The effect ofselenium deficiency on Cardiac morphology,biochemical index 1 1．1．2The researchprogressofcardiacapoptosisinducedby seleniumdeficiency ．2 1．2 The summarize ofmir-30b ．．3 1．2．1 MicroRNA biosynthesis 3 1．2．2 Microl；t】j{A mechanism ．．4 1．2．3 The research progess ofmir-30b ．．4 1．3 The research progess ofOxidation Resistance 1(Oxrl) ．．5 1．4 The objective and meaning ofthe research ．6 2 Tl僵ateriaI and method ．7 2．1 Instrument and reagent 一7 2．1．1 Instrument ． ． ．．7 2．1．2 Reagent ．．7 2．2 Method ． ． ． ． ． ．8 2．2．1 Experiment animals and grouping 8 2．2．2 The ultrastructure ofchicken cardiac muscle ．8 2．2．3 Mir-30b expression level in cardiac muscle by qPCR 9 2．2．4 Caspase3，Bax,Bcl一2，Oxrl expression levels in cardiac muscle by qPCR 12 2．2．5 Oxidative damage index detection in chicken cardiac muscle 13 2．2．6 The euture ofcardiac muscle cells ．13 2．2．7 The predicton oftarget gene ofmir-30b ．13 2．2．8 The construction ofmir-30b mimic and inhibitor in cardiac muscle cells 14 2．2．9 The construction ofpMIR-REPORT-Oxrlwith Oxrl 3’UTR(WT) 一15 2．2．10 The construction ofpMIR-REPORT-Oxrlwith Oxrl 3’UTR(MT) 17 2．2．1 l Luciferase reporter experiment ．17 2．2．12 Apoptosis levels in cells examined by TL兀怔L． 18 2．3 Analysis and statistics ．．18 3 Results ．1 9 3．1 The observation ofthe ultra．structure ofcardiac muscle 19 3．2 111e result ofmir-30b and Oxrl expression in selenium deficiency chicken cardiac muscle 19 3．3 The apoptosis gene expression levels in cardiac muscle 20 3．4 The result ofoxidative damage index detection in chicken cardiac muscle 一21 III 万方数据  东北农业大学农学硕士学位论文 3．4．1 The activity ofGPx、CAT、GSH、SOD in cardiac muscle ．21 3．4．2 The levels ofNO、iNOS in cardiac muscle ．22 3．4．3 The levels of H202 and OH in cardiac muscle ．23 3．4．4 The levels ofMDA in cardiac muscle ．23 3．5 The construction ofmir-