HNFA和FOXA2过表达诱导大鼠骨髓间充质干细胞定向分化为肝样细胞的研究-生物学·细胞生物学专业毕业论文.docx

ABSTRACTHepatocytes，which ABSTRACT Hepatocytes，which are the main performance of liver physiological function，arc widely used in basic research，drug development，as well in clinical application for patients with some liver diseases． However,the shortage of applicable hepatocytes severely restricts the research progress related、Ⅳitll hepatocyte，SO to hinder its process oftransformation and application in the clinical．Therefore，to explore the way how to get large number of pure，healthy and qualified hepatocytes of great importance．Due to the isolated primary hepatocytes relatively difficult to culture and donor livers are limited，SO it has been focusing inducing hepatocytes from other type cells which are easy to culture．In recent years， researchers have found that mesenchymal stem cells(MSCs)can be induced to variety ofcells germ layer．The induction of MSCs to hepatocyte-like has been received special attention by researchers，what’S more，this research provides new kind of seed cells for the treatment of liver disease and drug screening． Especially,bone marrow mesenchymal stem cell(BMSCs)have characteristics of self-renewal，highly malleable and immunomodulatory．In addition，the isolation and culture of BMSCs／n vitro is simple， exogenous gene Can be transferred and express in BMSCs easily．It also has the potential capability to be induced to variety of cells crOSS germ layer． FOXA2 and HNFlA important members of the hepatocyte nuclear factor(I-rNF)family,they abundantly expressed in the liver，and play important role in the differentiation and function of the liver cells．Based these studies，we hypothesized that FOXA2 and HNFlA could directly induce BMSCs to functional hepatocytes．Five days after the two factors were transduced to cells，we could observe some changes in cell morphology，pick out the cells with red fluorescent protein and the cell colonies with changed shape。RT-PCR,western blotting and immunofluorescence were used to verify whether the FOXA2+and Ⅲ 万方数据 HNFlA+