Ferroplasma thermophilum L1的分离 鉴定及应用于黄矿浸出的研究.docx

优秀毕业论文 精品参考文献资料 硕士学位论文 摘要 于pH，Fe3+的浓度可能是黄钾铁矾形成的更重要原因。 利用实时荧光定量PCR技术对浸矿微生物种群的分析表明，在浸 矿初期喜温硫杆菌杆菌和嗜铁钩端螺旋菌占较大的比例，但是到了浸 矿后期嗜铁钩端螺旋菌在种群中的比例明显降低。F thermophilum在 浸矿前期占据很少的数量，而到浸矿后期在种群中占据较大比例。嗜 酸硫化杆菌在种群中的比例没有太大变化。实验结果也是与它们的生 理生化特性相适应的。 关键词Ferroplasma thermophilum，生物浸出，黄铜矿，实时定量PCR， 种群动态 lI  硕士学位论文 ABSTRAC T Bioleaching iS all economical method for the recovery of metals from minerals，especially from low grade ores，overburden and waste from current mining operations，which requires moderate capital investment with low operating cost．Furthermore，bioleaching are environmentally friendly．In the present study,an extremely acidophilic and moderately thermophilic iron-oxidizing archaeon was isolated and characterized，and the strain represents a new species of genus Ferroplasma．To understand the role iron—oxidizing archaeon plays in mineral bioleaching,according to its physiological characteristics we focused on the effects of variously defined combinations of At．caldus，L． ferriphilum，acidophilus and E thermophilum on copper dissolution from chalcopyrite．The variation of pH，ferrous iron，ferric iron and copper was also investigated．The real-time quantitative PCR was applied to investigate the population dynamics of moderate thermophiles during bioleaching and the bioleaching mechanisms of chaicopyrite by the above microorganisms were discussed． A ferrous iron-oxidizing archaeon，named L 1，was isolated from a chalcopyrite-·leaching bioreactor．Strain L 1 is a non--motile COCCUS that lacks cell wall．The diameter of strain L 1 ranges from 0．4 to 1．0Ⅲn． ’Strain L 1 has a temperature optimum of 45 oC and the optimum initial pH for growth is 1．0．The generation time of strain L1 is 6．65 h．Strain L1 is capable of growth on ferrous iron and yeast extract．No growth occurred when ferrous iron or yeast extract presented as the sole energy source． The strain Can not grow on following organic substrates：peptone，glucose， lactose and galactose．Str