IMDC负载P2 5873段干预对EAN中Th17Th1细胞的影响.docx

硕士学位论文 中文摘要 结果： 1．树突状细胞的体外培养 培养6天的DC，流式细胞仪表型分析示CDl lc+ (66．13％--．10．81％)、MHC—IIl。w(40．03％±7．33％)CD8010w (24．72％+4．13％)、和CD8610w(18．6l％±3．68％)，证实所培养的细 胞是iMDC。 2． 动物模型： P258．73aa—iMDC干预组的临床症状较致病组明显减轻。 与致病组比较，P258．73aa．iMDC干预组的抗原特异性淋巴细胞增 殖反应显著降低(PO．01)。 P258．73aa．iMDC干预组的坐骨神经的炎性细胞浸润和脱髓鞘改变 较致病组明显减轻。 3．IL-17、RO脚和IFN．丫mRNA的表达 与佐剂对照组比较，致病组、iMDC干预组和P258．73aa．iMDC干 预组的IL．17、IFN．丫mRNA在脾脏、淋巴结和坐骨神经中的表达均 显著升高(PO．01)；与致病组比较，P258．73aa—iMDC干预组显著降低 (PO．01)。 脾脏和淋巴结中的ROR竹mRNA在各组的表达差异大致同IL-17 和IFN吖，但坐骨神经中，RO脚mRNA几乎无表达。 结论： 1．IL．17、ROR饥和IFN一丫表达上调可能促进EAN发病。 n  硕士学位论文 中文摘要 2．Thl7／Thl细胞可能协同诱导EAN的发生与发展。 3．P258．73aa．iMDC通过抗原特异性地抑制Thl7／Thl细胞而减轻 EAN的发病，可能是其诱导免疫耐受的机制之一。 关键词：实验性自身免疫性神经炎，Thl7细胞，Thl细胞，iMDC， 免疫耐受 III  硕十学位论文 英文摘要 ABSTRACT Objective To observe the expression of Th l 7 type cytokine IL一1 7、 the transcription factor of Th l 7 cells--ROR3,t and Th l type cytohne IFN一丫mRNA in spleen、lymph node and sciatic nerves at the maximum of EAN；To investigate the effect of iMDC pulsed with P258．73aa peptide (P258．73aa-iMDC)on Th l 7／Th l cells，explore the antigen specific tolerance mechanism of P258．73aa-iMDC in EAN． Method 1．Dendritic precursor cells from mouse bone marrow cultured and differentiated in medium supplemented with GM—CSF and IL-1 0．iMDC were collected at day 6 and pulsed with 50}tg／ml P258．73aa before use． 2．28 adult female Lewis mice were randomly enrolled into the adjuvant--control group， EAN group， iMDC treatment group and P258．73aa—iMDC treatment group，The EAN rats were immunized by injection into both hind footpads of inoculums containing 1 00嵋of P253．78aa peptide and CFA while the adjuvant-control rats were immunized with CFA only．In addition to immunization，P258．73aa—iMDC treatment groups were injected s．C P258．73aa．iMDC(5x 1 06／l m1)7 days before immunization while iMDC treatment groups were injected S．C iMDC． Mice were evaluated for clinical score before the experiment terminated at at the maximum of disease． IV  硕士学位论文 英文摘要 3．After the termination of exepfiment，hist